Adenozilkobinamid-fosfat guanililtransferaza

Adenozilkobinamid-fosfat guanililtransferaza
Identifikatori
EC broj	2.7.7.62
CAS broj	169592-55-6
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC članci PubMed članci NCBI proteini

Adenozilkobinamid-fosfat guanililtransferaza (EC 2.7.7.62, CobU, adenozilkobinamid kinaza/adenozilkobinamid-fosfat guanililtransferaza, AdoCbi kinaza/AdoCbi-fosfat guanililtransferaza) je enzim sa sistematskim imenom GTP:adenozilkobinamid-fosfat guanililtransferaza.^{[1][2][3][4][5]} Ovaj enzim katalizuje sledeću hemijsku reakciju

GTP + adenozilkobinamid fosfat ${\displaystyle \rightleftharpoons }$ difosfat + adenozilkobinamid-GDP

Kod Salmonella typhimurium LT2 pod anaerobnim uslovima, CobU (EC 2.7.7.62 i EC 2.7.1.156), CobT (EC 2.4.2.21), CobC (EC 3.1.3.73) i CobS (EC 2.7.8.26) katalizuju reakcije kojima se formira nukleotidna petlja, čime se konvertuje adenozilkobinamid (AdoCbi) u adenozilkobalamin (AdoCbl).

Reference

1. O'Toole, G.A. & Escalante-Semerena, J.C. (1995). „Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediate”. J. Biol. Chem. 270: 23560—23569. PMID 7559521. 
2. Thompson, T.B., Thomas, M.G., Escalante-Semerena, J.C. and Rayment, I. (1998). „Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3 Å resolution”. Biochemistry. 37: 7686—7695. PMID 9601028. CS1 одржавање: Вишеструка имена: списак аутора (веза)
3. Thompson, T.B., Thomas, M.G., Escalante-Semerena, J.C. and Rayment, I. (1999). „Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site”. Biochemistry. 38: 12995—13005. PMID 10529169. CS1 одржавање: Вишеструка имена: списак аутора (веза)
4. Thomas, M.G., Thompson, T.B., Rayment, I. and Escalante-Semerena, J.C. (2000). „Analysis of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) enzyme of Salmonella typhimurium LT2. Identification of residue His-46 as the site of guanylylation”. J. Biol. Chem. 275: 27576—27586. PMID 10869342. CS1 одржавање: Вишеструка имена: списак аутора (веза)
5. Warren, M.J., Raux, E., Schubert, H.L. and Escalante-Semerena, J.C. (2002). „The biosynthesis of adenosylcobalamin (vitamin B₁₂)”. Nat. Prod. Rep. 19: 390—412. PMID 12195810. CS1 одржавање: Вишеструка имена: списак аутора (веза)

Literatura

• Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036. 
• Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097. 

Spoljašnje veze

• Adenosylcobinamide-phosphate+guanylyltransferase на US National Library of Medicine Medical Subject Headings (MeSH)