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4-hidroksi-3-metilbut-2-enil difosfat reduktaza
4-hidroksi-3-metilbut-2-enil difosfat reduktaza
Identifikatori
EC broj	1.17.1.2
CAS broj	512789-14-9
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
PMC
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4-hidroksi-3-metilbut-2-enil difosfat reduktaza (EC 1.17.1.2, izopentenil-difosfat:NADP⁺ oksidoreduktaza, LytB, (E)-4-hidroksi-3-metilbut-2-en-1-il difosfatna reduktaza, HMBPP reduktaza, IspH, LytB/IspH) je enzim sa sistematskim imenom izopentenil-difosfat:NAD(P)⁺ oksidoreduktaza.^[1]^[2]^[3]^[4]^[5]^[6] Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) izopentenil difosfat + NAD(P)⁺ + H₂O

\rightleftharpoons

(E)-4-hidroksi-3-metilbut-2-en-1-il difosfat + NAD(P)H + H⁺

(2) dimetilalil difosfat + NAD(P)⁺ + H₂O

\rightleftharpoons

(E)-4-hidroksi-3-metilbut-2-en-1-il difosfat + NAD(P)H + H⁺

Ovaj enzim je gvožđe-sumporni protein koji sadrži bilo [3Fe-4S] ili [4Fe-4S] kluster. Enzim sačinjava sistem u kome se feredoksin prvo redukuje i naknadno se reoksiduje posredstvom NAD(P)⁺-zavisne reduktaze.

Reference

^ Rohdich, F., Hecht, S., Gärtner, K., Adam, P., Krieger, C., Amslinger, S., Arigoni, D., Bacher, A. and Eisenreich, W. (2002). „Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein”. Proc. Natl. Acad. Sci. USA. 99: 1158—1163. PMID 11818558.
^ Hintz, M., Reichenberg, A., Altincicek, B., Bahr, U., Gschwind, R.M., Kollas, A.-K., Beck, E., Wiesner, J., Eberl, M. and Jomaa, H. (2001). „Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human T cells in Escherichia coli”. FEBS Lett. 509: 317—322. PMID 11741609.
^ Charon, L., Pale-Grosdemange, C. and Rohmer, M. (1999). „On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis”. Tetrahedron Lett. 40: 7231—7234.
^ Röhrich, R.C., Englert, N., Troschke, K., Reichenberg, A., Hintz, M., Seeber, F., Balconi, E., Aliverti, A., Zanetti, G., Köhler, U., Pfeiffer, M., Beck, E., Jomaa, H. and Wiesner, J. (2005). „Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum”. FEBS Lett. 579: 6433—6438. PMID 16289098.
^ Wolff, M., Seemann, M., Bui, T.S.B., Frapart, Y., Tritsch, D., Garcia Estrabot, A., Rodríguez-Concepción, M., Boronat, A., Marquet, A. and Rohmer, M. (2003). „Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein”. FEBS Lett. 541: 115—120. PMID 12706830.
^ Gräwert, T., Kaiser, J., Zepeck, F., Laupitz, R., Hecht, S., Amslinger, S., Schramek, N., Schleicher, E., Weber, S., Haslbeck, M., Buchner, J., Rieder, C., Arigoni, D., Bacher, A., Eisenreich, W. and Rohdich, F. (2004). „IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis”. J. Am. Chem. Soc. 126: 12847—12855. PMID 15469281.

Literatura

Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.

Spoljašnje veze

4-hydroxy-3-methylbut-2-enyl+diphosphate+reductase на US National Library of Medicine Medical Subject Headings (MeSH)

[1] Rohdich, F., Hecht, S., Gärtner, K., Adam, P., Krieger, C., Amslinger, S., Arigoni, D., Bacher, A. and Eisenreich, W. (2002). „Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein”. Proc. Natl. Acad. Sci. USA. 99: 1158—1163. PMID 11818558.

[2] Hintz, M., Reichenberg, A., Altincicek, B., Bahr, U., Gschwind, R.M., Kollas, A.-K., Beck, E., Wiesner, J., Eberl, M. and Jomaa, H. (2001). „Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human T cells in Escherichia coli”. FEBS Lett. 509: 317—322. PMID 11741609.

[3] Charon, L., Pale-Grosdemange, C. and Rohmer, M. (1999). „On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis”. Tetrahedron Lett. 40: 7231—7234.

[4] Röhrich, R.C., Englert, N., Troschke, K., Reichenberg, A., Hintz, M., Seeber, F., Balconi, E., Aliverti, A., Zanetti, G., Köhler, U., Pfeiffer, M., Beck, E., Jomaa, H. and Wiesner, J. (2005). „Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum”. FEBS Lett. 579: 6433—6438. PMID 16289098.

[5] Wolff, M., Seemann, M., Bui, T.S.B., Frapart, Y., Tritsch, D., Garcia Estrabot, A., Rodríguez-Concepción, M., Boronat, A., Marquet, A. and Rohmer, M. (2003). „Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein”. FEBS Lett. 541: 115—120. PMID 12706830.

[6] Gräwert, T., Kaiser, J., Zepeck, F., Laupitz, R., Hecht, S., Amslinger, S., Schramek, N., Schleicher, E., Weber, S., Haslbeck, M., Buchner, J., Rieder, C., Arigoni, D., Bacher, A., Eisenreich, W. and Rohdich, F. (2004). „IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis”. J. Am. Chem. Soc. 126: 12847—12855. PMID 15469281.

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4-Hidroksi-3-metilbut-2-enil difosfat reduktaza

Reference

Literatura

Spoljašnje veze