TRNK (adenin57-N1/adenin58-N1)-metiltransferaza

TRNK (adenin^57-N^1/adenin^58-N^1)-metiltransferaza
Identifikatori
EC broj	2.1.1.219
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga PMC articles PubMed articles NCBI Protein search

TRNK (adenin⁵⁷-N¹/adenin⁵⁸-N¹)-metiltransferaza (EC 2.1.1.219, TrmI, PabTrmI, AqTrmI, MtTrmI) je enzim sa sistematskim imenom S-adenozil-L-metionin:tRNK (adenin⁵⁷/adenin⁵⁸-N¹)-metiltransferaza.^[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju

2 S-adenozil-L-metionin + adenin⁵⁷/adenin⁵⁸ u tRNK ${\displaystyle \rightleftharpoons }$ 2 S-adenozil-L-homocistein + N¹-metiladenin⁵⁷/N¹-metiladenin⁵⁸ u tRNK

Ovaj enzim katalizuje formiranje N¹-metiladenina u dve susedne pozicije (57 i 58) u T-petlji pojedinih tRNK molekula (e.g. tRNKAsp). Metiladenozin u poziciji 57 je obligatorni intermedijer za sintezu metilinozina, koji je obično prisutan u poziciji 57 arhejnih tRNK molekula.

Reference

1. Roovers, M., Wouters, J., Bujnicki, J.M., Tricot, C., Stalon, V., Grosjean, H. and Droogmans, L. (2004). „A primordial RNA modification enzyme: the case of tRNA (m¹A) methyltransferase”. Nucleic Acids Res. 32: 465-476. PMID 14739239. 
2. Guelorget, A., Roovers, M., Guerineau, V., Barbey, C., Li, X. and Golinelli-Pimpaneau, B. (2010). „Insights into the hyperthermostability and unusual region-specificity of archaeal Pyrococcus abyssi tRNA m¹A^57/58 methyltransferase”. Nucleic Acids Res. 38: 6206-6218. PMID 20483913. 

Literatura

• Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
• Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
• Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
• Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
• Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
• Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Vanjske veze

• MeSH TRNA+(adenine57-N1/adenine58-N1)-methyltransferase