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Proteinska tirozinska kinaza 6
	; NMR struktura SH2 domena PTK6.
Dostupne strukture
	1RJA, 2KGT
Identifikatori
Simboli	PTK6; BRK
Vanjski ID	OMIM: 602004 MGI: 99683 HomoloGene: 68494 GeneCards: PTK6 Gene
EC broj	2.7.10.2
Ontologija gena
Molekulska funkcija	• aktivnost proteinske serin/treoninske kinaze; • aktivnost nemembranske proteinske tirozinske kinaze; • proteinsko vezivanje;
Ćelijska komponenta	• nukleus; • nukleolus; • citoplazma; • membrana;
Biološki proces	• proteinska fosforilacija; • pozitivna regulacija razvića neuronskih projekcija; • ćelijska migracija;
Pregled RNK izražavanja
	podaci
Ortolozi

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Tirozinska proteinska kinaza 6 je enzim koji je kod ljudi kodiran PTK6 genom.^[2]^[3]^[4]

Funkcija

Tirozinska proteinska kinazae 6 (takođe poznata kao kinaza tumora dojke, Brk) je citoplazmna nereceptorska proteinska kinaza koja deluje kao intraćelijski prenosnik signala u epitelnim tkivima. Ovaj protein je podložan autofosforilaciji.^[4]

Klinički značaj

Prekomerno izražavanja ovog gena u epitelnim ćelijama sisara dovodi do senzitizacije ćelija na epidermalni faktor rasta, što dovodi do parcijalno transformisanog fenotipa. Izražavanje ovog gena je detektovano u niskim nivoima u pojedinim tumorima dojke, do čega ne dolazi u normalnom tkivu dojke.^[4]

Interactions

PTK6 formira interakcije sa STAP2^[5] i KHDRBS1.^[6]

Reference

↑ PDB 1rja; Hong E, Shin J, Kim HI, Lee ST, Lee W (July 2004). „Solution structure and backbone dynamics of the non-receptor protein-tyrosine kinase-6 Src homology 2 domain”. J. Biol. Chem. 279 (28): 29700–8. DOI:10.1074/jbc.M313185200. PMID 15056653.
↑ Lee ST, Strunk KM, Spritz RA (December 1993). „A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes”. Oncogene 8 (12): 3403–10. PMID 8247543.
↑ Park SH, Lee KH, Kim H, Lee ST (September 1997). „Assignment of the human PTK6 gene encoding a non-receptor protein tyrosine kinase to 20q13.3 by fluorescence in situ hybridization”. Cytogenet Cell Genet 77 (3–4): 271–2. DOI:10.1159/000134595. PMID 9284935.
↑ ^4,0 ^4,1 ^4,2 „Entrez Gene: PTK6 PTK6 protein tyrosine kinase 6”.
↑ Mitchell, P J; Sara E A; Crompton M R (August 2000). „A novel adaptor-like protein which is a substrate for the non-receptor tyrosine kinase, BRK”. Oncogene (ENGLAND) 19 (37): 4273–82. DOI:10.1038/sj.onc.1203775. ISSN 0950-9232. PMID 10980601.
↑ Derry, J J; Richard S; Valderrama Carvajal H; Ye X; Vasioukhin V; Cochrane A W; Chen T; Tyner A L (August 2000). „Sik (BRK) Phosphorylates Sam68 in the Nucleus and Negatively Regulates Its RNA Binding Ability”. Mol. Cell. Biol. (UNITED STATES) 20 (16): 6114–26. DOI:10.1128/MCB.20.16.6114-6126.2000. ISSN 0270-7306. PMC 86087. PMID 10913193.

Literatura

Serfas MS, Tyner AL (2003). „Brk, Srm, Frk, and Src42A form a distinct family of intracellular Src-like tyrosine kinases”. Oncol. Res. 13 (6–10): 409–19. PMID 12725532.
Mitchell PJ, Barker KT, Martindale JE, et al. (1994). „Cloning and characterisation of cDNAs encoding a novel non-receptor tyrosine kinase, brk, expressed in human breast tumours”. Oncogene 9 (8): 2383–90. PMID 8036022.
Kamalati T, Jolin HE, Mitchell PJ, et al. (1997). „Brk, a breast tumor-derived non-receptor protein-tyrosine kinase, sensitizes mammary epithelial cells to epidermal growth factor”. J. Biol. Chem. 271 (48): 30956–63. DOI:10.1074/jbc.271.48.30956. PMID 8940083.
Lee H, Kim M, Lee KH, et al. (1998). „Exon-intron structure of the human PTK6 gene demonstrates that PTK6 constitutes a distinct family of non-receptor tyrosine kinase”. Mol. Cells 8 (4): 401–7. PMID 9749526.
Llor X, Serfas MS, Bie W, et al. (1999). „BRK/Sik expression in the gastrointestinal tract and in colon tumors”. Clin. Cancer Res. 5 (7): 1767–77. PMID 10430081.
Derry JJ, Richard S, Valderrama Carvajal H, et al. (2000). „Sik (BRK) Phosphorylates Sam68 in the Nucleus and Negatively Regulates Its RNA Binding Ability”. Mol. Cell. Biol. 20 (16): 6114–26. DOI:10.1128/MCB.20.16.6114-6126.2000. PMC 86087. PMID 10913193.
Mitchell PJ, Sara EA, Crompton MR (2000). „A novel adaptor-like protein which is a substrate for the non-receptor tyrosine kinase, BRK”. Oncogene 19 (37): 4273–82. DOI:10.1038/sj.onc.1203775. PMID 10980601.
Kamalati T, Jolin HE, Fry MJ, Crompton MR (2000). „Expression of the BRK tyrosine kinase in mammary epithelial cells enhances the coupling of EGF signalling to PI 3-kinase and Akt, via erbB3 phosphorylation”. Oncogene 19 (48): 5471–6. DOI:10.1038/sj.onc.1203931. PMID 11114724.
Hong E, Shin J, Bang E, et al. (2001). „Complete sequence-specific 1H, 13C and 15N resonance assignments of the human PTK6 SH2 domain”. J. Biomol. NMR 19 (3): 291–2. DOI:10.1023/A:1011221125013. PMID 11330822.
Deloukas P, Matthews LH, Ashurst J, et al. (2002). „The DNA sequence and comparative analysis of human chromosome 20”. Nature 414 (6866): 865–71. DOI:10.1038/414865a. PMID 11780052.
Kang KN, Kim M, Pae KM, Lee ST (2002). „Characterization of the 5'-flanking region of the human PTK6 gene”. Biochim. Biophys. Acta 1574 (3): 365–9. DOI:10.1016/s0167-4781(02)00234-8. PMID 11997104.
Qiu H, Miller WT (2002). „Regulation of the nonreceptor tyrosine kinase Brk by autophosphorylation and by autoinhibition”. J. Biol. Chem. 277 (37): 34634–41. DOI:10.1074/jbc.M203877200. PMID 12121988.
Koo BK, Kim MH, Lee ST, Lee W (2002). „Purification and spectroscopic characterization of the human protein tyrosine kinase-6 SH3 domain”. J. Biochem. Mol. Biol. 35 (3): 343–7. DOI:10.5483/BMBRep.2002.35.3.343. PMID 12297019.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences”. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. DOI:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Coyle JH, Guzik BW, Bor YC, et al. (2003). „Sam68 Enhances the Cytoplasmic Utilization of Intron-Containing RNA and Is Functionally Regulated by the Nuclear Kinase Sik/BRK”. Mol. Cell. Biol. 23 (1): 92–103. DOI:10.1128/MCB.23.1.92-103.2003. PMC 140664. PMID 12482964.
Derry JJ, Prins GS, Ray V, Tyner AL (2003). „Altered localization and activity of the intracellular tyrosine kinase BRK/Sik in prostate tumor cells”. Oncogene 22 (27): 4212–20. DOI:10.1038/sj.onc.1206465. PMID 12833144.
Qiu H, Miller WT (2004). „Role of the Brk SH3 domain in substrate recognition”. Oncogene 23 (12): 2216–23. DOI:10.1038/sj.onc.1207339. PMID 14676834.

[pmid15056653-1] PDB 1rja; Hong E, Shin J, Kim HI, Lee ST, Lee W (July 2004). „Solution structure and backbone dynamics of the non-receptor protein-tyrosine kinase-6 Src homology 2 domain”. J. Biol. Chem. 279 (28): 29700–8. DOI:10.1074/jbc.M313185200. PMID 15056653.

[pmid8247543-2] Lee ST, Strunk KM, Spritz RA (December 1993). „A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes”. Oncogene 8 (12): 3403–10. PMID 8247543.

[pmid9284935-3] Park SH, Lee KH, Kim H, Lee ST (September 1997). „Assignment of the human PTK6 gene encoding a non-receptor protein tyrosine kinase to 20q13.3 by fluorescence in situ hybridization”. Cytogenet Cell Genet 77 (3–4): 271–2. DOI:10.1159/000134595. PMID 9284935.

[entrez-4] 4,0 ^4,1 ^4,2 „Entrez Gene: PTK6 PTK6 protein tyrosine kinase 6”.

[pmid10980601-5] Mitchell, P J; Sara E A; Crompton M R (August 2000). „A novel adaptor-like protein which is a substrate for the non-receptor tyrosine kinase, BRK”. Oncogene (ENGLAND) 19 (37): 4273–82. DOI:10.1038/sj.onc.1203775. ISSN 0950-9232. PMID 10980601.

[pmid10913193-6] Derry, J J; Richard S; Valderrama Carvajal H; Ye X; Vasioukhin V; Cochrane A W; Chen T; Tyner A L (August 2000). „Sik (BRK) Phosphorylates Sam68 in the Nucleus and Negatively Regulates Its RNA Binding Ability”. Mol. Cell. Biol. (UNITED STATES) 20 (16): 6114–26. DOI:10.1128/MCB.20.16.6114-6126.2000. ISSN 0270-7306. PMC 86087. PMID 10913193.

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