ZP3

ZP3
Identifiers
Aliases	ZP3, ZP3A, ZP3B, ZPC, Zp-3, zona pellucida glycoprotein 3 (sperm receptor), zona pellucida glycoprotein 3, OOMD3
External IDs	OMIM: 182889; MGI: 99215; HomoloGene: 5178; GeneCards: ZP3; OMA:ZP3 - orthologs
Gene location (Human) Chr. Chromosome 7 (human)[1] Band 7q11.23 Start 76,397,518 bp[1] End 76,442,071 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5 G2|5 75.62 cM Start 136,008,953 bp[2] End 136,017,478 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in gonad testicle apex of heart mucosa of transverse colon bone marrow cells ventricular zone muscle tissue body of pancreas right adrenal cortex nucleus accumbens Top expressed in primary oocyte secondary oocyte zygote egg cell embryo morula morula embryo ovary blastocyst More reference expression data BioGPS n/a
Gene ontology Molecular function acrosin binding protein binding carbohydrate binding identical protein binding receptor ligand activity extracellular matrix structural constituent structural constituent of egg coat Cellular component integral component of membrane membrane extracellular matrix plasma membrane extracellular region extracellular space intracellular anatomical structure collagen-containing extracellular matrix Biological process positive regulation of inflammatory response positive regulation of leukocyte migration positive regulation of phosphatidylinositol biosynthetic process positive regulation of antral ovarian follicle growth positive regulation of interferon-gamma production blastocyst formation positive regulation of interleukin-4 production oocyte development positive regulation of type IV hypersensitivity positive regulation of transcription, DNA-templated humoral immune response mediated by circulating immunoglobulin positive regulation of acrosome reaction positive regulation of humoral immune response positive regulation of T cell proliferation binding of sperm to zona pellucida negative regulation of binding of sperm to zona pellucida positive regulation of ovarian follicle development positive regulation of acrosomal vesicle exocytosis negative regulation of transcription, DNA-templated phosphatidylinositol-mediated signaling single fertilization egg coat formation regulation of signaling receptor activity Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7784 22788 Ensembl ENSG00000188372 ENSMUSG00000004948 UniProt P21754 P10761 RefSeq (mRNA) NM_007155 NM_001110354 NM_011776 RefSeq (protein) NP_001103824 NP_009086 NP_035906 Location (UCSC) Chr 7: 76.4 – 76.44 Mb Chr 5: 136.01 – 136.02 Mb PubMed search [3] [4]
Wikidata
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Zona pellucida sperm-binding protein 3, also known as zona pellucida glycoprotein 3 (Zp-3) or the sperm receptor, is a ZP module-containing protein that in humans is encoded by the ZP3 gene.^[5] ZP3 is the glycoprotein in the zona pellucida most important for inducting the acrosome reaction of sperm cells at the beginning of fertilization.^[6]

Function

The zona pellucida (ZP) is a specialized extracellular matrix that surrounds the oocyte and early embryo. It is composed of three or four glycoproteins (ZP1-4) with various functions during oogenesis, fertilization and preimplantation development. The protein encoded by this gene is a major structural component of the ZP and functions in primary binding and stimulation of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved "ZP domain" module, a consensus furin cleavage site (CFCS), a polymerization-blocking external hydrophobic patch (EHP), and a C-terminal transmembrane domain. Cleavage at the CFCS separates the mature protein from the EHP, allowing it to incorporate into nascent ZP filaments. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.^[5]

In mice, ZP3 (more specifically the portion in its exon 7) is the single ZP protein that is sufficient and necessary for sperm binding in vitro,^[6] but is insufficient for fertilization in vivo.^[7] In humans, ZP1, ZP3, and ZP4 all appear partially responsible for starting the acrosome reaction.^[8]

Orthologs of these genes are found throughout Vertebrata. The western clawed frog appears to have two orthologs, and the sea lamprey has seven.^[9]

3D Structure

X-ray crystallographic studies of the N-terminal half of mammalian ZP3 (PDB: 3D4C, 3D4G, 3EF7, 5OSQ​)^[10] as well as its full-length avian homolog (PDB: 3NK3, 3NK4​)^[11] revealed that the protein's ZP module consists of two immunoglobulin-like domains, ZP-N and ZP-C. The latter, which contains EHP as well as a ZP3-specific subdomain, interacts with the ZP-N domain of a second molecule to generate an antiparallel homodimeric arrangement required for protein secretion.^[11]

Mutations

The Zona Pellucida (ZP) is a complex matrix of glycoprotein that surrounds the oocyte and plays a crucial role in the attachment of sperm during reproduction. Ultimately, through the facilitation of sperm binding and the initiation of the acrosome reaction, the ZP proteins are essential to reproduction and have an important impact on fertility. Research through the Journal of Cellular and Molecular Medicine conducted experiments to determine the mechanisms surrounding possible mutations to the ZP gene and how they would impact fertility.^[12] By performing whole-exome sequencing they isolated a genome that had a mutation in the ZP3 and the ZP1 genes. They then transfected these genes into HeLa cell cultures and ran a variety of tests to isolate the consequences of these mutations. The authors wrote this regarding their results:

“The results indicate that the mutations are involved in the reduced secretion of ZP1 and ZP3 and leading to connection failure of the ZP filaments in vitro. The data suggest a potential that the mutations may be involved in the lacking ZP phenotype, which need to be further investigated in vivo.” (Cao, Qiqi, et al.)

It is clear that the ZP proteins are crucial to expressing a correct ZP phenotype in humans, in which all of the ZP proteins 1-4 are properly functioning. Without this interface of proper protein function, sperm binding is inhibited, and fertility is compromised.

References

1. GRCh38: Ensembl release 89: ENSG00000188372 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000004948 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: zona pellucida glycoprotein 3 (sperm receptor)".
6. Litscher, E. S.; Williams, Z.; Wassarman, P. M. (2009). "Zona pellucida glycoprotein ZP3 and fertilization in mammals". Molecular Reproduction and Development. 76 (10): 933–941. doi:10.1002/mrd.21046. PMID 19504560. S2CID 21186053.
7. Avella MA, Baibakov B, Dean J (2014). "A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans". J Cell Biol. 205 (6): 801–809. doi:10.1083/jcb.201404025. PMC 4068139. PMID 24934154. Female mice that form a zona pellucida lacking ZP2 are sterile
8. Gupta, SK; Bhandari, B (January 2011). "Acrosome reaction: relevance of zona pellucida glycoproteins". Asian Journal of Andrology. 13 (1): 97–105. doi:10.1038/aja.2010.72. PMC 3739397. PMID 21042299.
9. "EggNOG Database | Orthology predictions and functional annotation". eggnogdb.embl.de. Archived from the original on 4 March 2019. Retrieved 5 March 2019.
10. Monné M, Han L, Schwend T, Burendahl S, Jovine L (2008). "Crystal structure of the ZP-N domain of ZP3 reveals the core fold of animal egg coats". Nature. 456 (7222): 653–7. Bibcode:2008Natur.456..653M. doi:10.1038/nature07599. hdl:11563/8930. PMID 19052627. S2CID 4430083. PDB: 3D4C, 3D4G, 3EF7​
11. Han L, Monné M, Okumura H, Schwend T, Cherry AL, Flot D, Matsuda T, Jovine L (2010). "Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3". Cell. 143 (3): 404–15. doi:10.1016/j.cell.2010.09.041. hdl:11563/8931. PMID 20970175. S2CID 18583237. PDB: 3NK3, 3NK4​
12. Cao, Qiqi; Zhao, Chun; Zhang, Xiaolan; Zhang, Heng; Lu, Qianneng; Wang, Congjing; Hu, Yue; Ling, Xiufeng; Zhang, Junqiang; Huo, Ran (2020-06-22). "Heterozygous mutations in ZP1 and ZP3 cause formation disorder of ZP and female infertility in human". Journal of Cellular and Molecular Medicine. 24 (15): 8557–8566. doi:10.1111/jcmm.15482. ISSN 1582-1838. PMC 7412702. PMID 32573113.

^[1]

Further reading

• Bansal P, Chakrabarti K, Gupta SK (2009). "Functional activity of human ZP3 primary sperm receptor resides toward its C-terminus". Biol. Reprod. 81 (1): 7–15. doi:10.1095/biolreprod.108.074716. PMID 19246320.
• Bleil JD, Wassarman PM (1980). "Mammalian sperm-egg interaction: identification of a glycoprotein in mouse egg zonae pellucidae possessing receptor activity for sperm". Cell. 20 (3): 873–82. doi:10.1016/0092-8674(80)90334-7. PMID 7418009. S2CID 29105880.
• Caballero-Campo P, Chirinos M, Fan XJ, et al. (2006). "Biological effects of recombinant human zona pellucida proteins on sperm function". Biol. Reprod. 74 (4): 760–8. doi:10.1095/biolreprod.105.047522. PMID 16407501.
• Chamberlin ME, Dean J (1990). "Human homolog of the mouse sperm receptor". Proc. Natl. Acad. Sci. U.S.A. 87 (16): 6014–8. Bibcode:1990PNAS...87.6014C. doi:10.1073/pnas.87.16.6014. PMC 54462. PMID 2385582.
• Chiu PC, Wong BS, Chung MK, et al. (2008). "Effects of native human zona pellucida glycoproteins 3 and 4 on acrosome reaction and zona pellucida binding of human spermatozoa". Biol. Reprod. 79 (5): 869–77. doi:10.1095/biolreprod.108.069344. PMID 18667750.
• Choudhury S, Ganguly A, Chakrabarti K, et al. (2009). "DNA vaccine encoding chimeric protein encompassing epitopes of human ZP3 and ZP4: immunogenicity and characterization of antibodies". J. Reprod. Immunol. 79 (2): 137–47. doi:10.1016/j.jri.2008.09.002. PMID 19004505.
• Dell A, Chalabi S, Easton RL, et al. (2003). "Murine and human zona pellucida 3 derived from mouse eggs express identical O-glycans". Proc. Natl. Acad. Sci. U.S.A. 100 (26): 15631–6. Bibcode:2003PNAS..10015631D. doi:10.1073/pnas.2635507100. PMC 307619. PMID 14673092.
• Florman HM, Wassarman PM (1985). "O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity". Cell. 41 (1): 313–24. doi:10.1016/0092-8674(85)90084-4. PMC 7133279. PMID 2986849.
• Furlong LI, Harris JD, Vazquez-Levin MH (2005). "Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC". Fertil. Steril. 83 (6): 1780–90. doi:10.1016/j.fertnstert.2004.12.042. PMID 15950651.
• Gook DA, Edgar DH, Borg J, Martic M (2008). "Detection of zona pellucida proteins during human folliculogenesis". Hum. Reprod. 23 (2): 394–402. doi:10.1093/humrep/dem373. PMID 18033806.
• Han L, Monné M, Okumura H, Schwend T, Cherry AL, Flot D, Matsuda T, Jovine L (2010). "Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3". Cell. 143 (3): 404–15. doi:10.1016/j.cell.2010.09.041. hdl:11563/8931. PMID 20970175. S2CID 18583237. PDB: 3NK3​ PDB: 3NK4​
• Jovine L, Qi H, Williams Z, Litscher E, Wassarman PM (2002). "The ZP domain is a conserved module for polymerization of extracellular proteins". Nat. Cell Biol. 4 (6): 457–61. doi:10.1038/ncb802. PMID 12021773. S2CID 11575790.
• Jovine L, Qi H, Williams Z, Litscher ES, Wassarman PM (2004). "A duplicated motif controls assembly of zona pellucida domain proteins". Proc. Natl. Acad. Sci. U.S.A. 101 (16): 5922–7. Bibcode:2004PNAS..101.5922J. doi:10.1073/pnas.0401600101. PMC 395899. PMID 15079052.
• Kiefer SM, Saling P (2002). "Proteolytic processing of human zona pellucida proteins". Biol. Reprod. 66 (2): 407–14. doi:10.1095/biolreprod66.2.407. PMID 11804956.
• Monné M, Han L, Schwend T, Burendahl S, Jovine L (2008). "Crystal structure of the ZP-N domain of ZP3 reveals the core fold of animal egg coats". Nature. 456 (7222): 653–7. Bibcode:2008Natur.456..653M. doi:10.1038/nature07599. hdl:11563/8930. PMID 19052627. S2CID 4430083. PDB: 3D4C​ PDB: 3D4G​ PDB: 3EF7​
• Ni Y, Li K, Xu W, et al. (2007). "Acrosome reaction induced by recombinant human zona pellucida 3 peptides rhuZP3a22 approximately 176 and rhuZP3b177 approximately 348 and their mechanism". J. Androl. 28 (3): 381–8. doi:10.2164/jandrol.106.001289. PMID 17192598.
• Qi H, Williams Z, Wassarman PM (2002). "Secretion and assembly of zona pellucida glycoproteins by growing mouse oocytes microinjected with epitope-tagged cDNAs for mZP2 and mZP3". Mol. Biol. Cell. 13 (2): 530–41. doi:10.1091/mbc.01-09-0440. PMC 65647. PMID 11854410.
• Rankin T, Dean J (2000). "The zona pellucida: using molecular genetics to study the mammalian egg coat". Rev. Reprod. 5 (2): 114–21. doi:10.1530/ror.0.0050114. PMID 10864856.
• Törmälä RM, Jääskeläinen M, Lakkakorpi J, et al. (2008). "Zona pellucida components are present in human fetal ovary before follicle formation" (PDF). Mol. Cell. Endocrinol. 289 (1–2): 10–5. doi:10.1016/j.mce.2008.01.029. PMID 18502569. S2CID 3455166.
• van Duin M, Polman JE, Verkoelen CC, Bunschoten H, Meyerink JH, Olijve W, Aitken RJ (December 1992). "Cloning and characterization of the human sperm receptor ligand ZP3: evidence for a second polymorphic allele with a different frequency in the Caucasian and Japanese populations". Genomics. 14 (4): 1064–70. doi:10.1016/S0888-7543(05)80130-2. PMID 1478648.
• Wassarman PM, Jovine L, Litscher ES (2001). "A profile of fertilization in mammals". Nat. Cell Biol. 3 (2): E59–64. doi:10.1038/35055178. PMID 11175768. S2CID 6172791.
• Zhao M, Boja ES, Hoodbhoy T, et al. (2004). "Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cells". Biochemistry. 43 (38): 12090–104. doi:10.1021/bi048958k. PMID 15379548.
• Zhao M, Gold L, Ginsberg AM, et al. (2002). "Conserved furin cleavage site not essential for secretion and integration of ZP3 into the extracellular egg coat of transgenic mice". Mol. Cell. Biol. 22 (9): 3111–20. doi:10.1128/MCB.22.9.3111-3120.2002. PMC 133755. PMID 11940668.

External links

• ZP3+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

1. Cao, Qiqi, et al. “Heterozygous Mutations in ZP1 and ZP3 Cause Formation ...” Journal of Cellular and Molecular Medicine, Wiley Online Library, 22 June 2020, onlinelibrary.wiley.com/doi/10.1111/jcmm.15482.