VPS25

Available protein structures:
PDB	IPR008570 PF05871 (ECOD; PDBsum)
AlphaFold	IPR008570; PF05871;

ESCRT-II complex subunit
ESCRT-II complex subunit
	crystal structure of subunit vps25 of the endosomal trafficking complex escrt-ii
Identifiers
Symbol	ESCRT-II
Pfam	PF05871
InterPro	IPR008570
PDB
Available protein structures:
PDB	IPR008570 PF05871 (ECOD; PDBsum)
AlphaFold	IPR008570; PF05871;

VPS25
Identifiers
Aliases	VPS25, DERP9, EAP20, FAP20, vacuolar protein sorting 25 homolog
External IDs	OMIM: 610907; MGI: 106354; GeneCards: VPS25
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	2ZME, 3CUQ, 3HTU
Gene location (Human)
Chr.	Chromosome 17 (human)
End	42,779,599 bp
Gene location (Mouse)
Chr.	Chromosome 11 (mouse)
End	101,150,375 bp
RNA expression pattern
	Top expressed in
	mucosa of transverse colon; ; rectum; ; islet of Langerhans; ; skin of arm; ; right uterine tube; ; mucosa of ileum; ; body of stomach; ; smooth muscle tissue; ; stromal cell of endometrium; ; olfactory zone of nasal mucosa;
	Top expressed in
	yolk sac; ; tail of embryo; ; genital tubercle; ; right kidney; ; lip; ; embryo; ; embryo; ; granulocyte; ; muscle of thigh; ; blastocyst;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	protein homodimerization activity; protein N-terminus binding; structural molecule activity; protein binding;
Cellular component	cytoplasm; cytosol; endosome; membrane; nucleoplasm; endosome membrane; extracellular exosome; nucleus; ESCRT II complex;
Biological process	regulation of transcription, DNA-templated; multivesicular body assembly; transcription, DNA-templated; endosomal transport; protein transport; protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; macroautophagy; negative regulation of epidermal growth factor-activated receptor activity; transport; multivesicular body sorting pathway;
	Sources:Amigo / QuickGO
Orthologs
Databases	NCBI: entry; OMA: entry
	84313
	28084
	ENSG00000131475
	ENSMUSG00000078656
	Q9BRG1
	Q9CQ80
	NM_032353
	NM_001284411; NM_001284412; NM_001284414; NM_026776
	NP_115729; NP_115729.1
	NP_001271340; NP_001271341; NP_001271343; NP_081052
	Wikidata
View/Edit Human	View/Edit Mouse

Vacuolar protein-sorting-associated protein 25 is a protein that in humans is encoded by the VPS25 gene.^[5]^[6]

It is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT (ESCRT-I, -II, -III) complexes orchestrate efficient sorting of ubiquitinated transmembrane receptors to lysosomes via multivesicular bodies (MVBs).^[7] ESCRT-II recruits the transport machinery for protein sorting at MVB.^[8] In addition, the human ESCRT-II has been shown to form a complex with RNA polymerase II elongation factor ELL in order to exert transcriptional control activity. ESCRT-II transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo.^[9]

ESCRT-II is a trilobal complex composed of two copies of vps25, one copy of vps22 and the C-terminal region of vps36. The crystal structure of vps25 revealed two winged-helix domains, the N-terminal domain of vps25 interacting with vps22 and vps36.^[10]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000131475 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000078656 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Yorikawa C, Shibata H, Waguri S, Hatta K, Horii M, Katoh K, Kobayashi T, Uchiyama Y, Maki M (Mar 2005). "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting". Biochem J. 387 (Pt 1): 17–26. doi:10.1042/BJ20041227. PMC 1134928. PMID 15511219.
^ "Entrez Gene: VPS25 vacuolar protein sorting 25 homolog (S. cerevisiae)".
^ Gill DJ, Teo H, Sun J, Perisic O, Veprintsev DB, Emr SD, Williams RL (January 2007). "Structural insight into the ESCRT-I/-II link and its role in MVB trafficking". EMBO J. 26 (2): 600–12. doi:10.1038/sj.emboj.7601501. PMC 1783442. PMID 17215868.
^ Teo H, Perisic O, Gonzalez B, Williams RL (October 2004). "ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes". Dev. Cell. 7 (4): 559–69. doi:10.1016/j.devcel.2004.09.003. PMID 15469844.
^ Babst M, Katzmann DJ, Snyder WB, Wendland B, Emr SD (August 2002). "Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body". Dev. Cell. 3 (2): 283–9. doi:10.1016/S1534-5807(02)00219-8. PMID 12194858.
^ Wernimont AK, Weissenhorn W (December 2004). "Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II". BMC Struct. Biol. 4 (1): 10. doi:10.1186/1472-6807-4-10. PMC 539351. PMID 15579210.

Kamura T, Burian D, Khalili H, et al. (2001). "Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose". J. Biol. Chem. 276 (19): 16528–33. doi:10.1074/jbc.M010142200. PMID 11278625.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
von Schwedler UK, Stuchell M, Müller B, et al. (2003). "The protein network of HIV budding". Cell. 114 (6): 701–13. doi:10.1016/S0092-8674(03)00714-1. PMID 14505570. S2CID 16894972.
Martin-Serrano J, Yarovoy A, Perez-Caballero D, et al. (2003). "Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins". Proc. Natl. Acad. Sci. U.S.A. 100 (21): 12414–9. Bibcode:2003PNAS..10012414M. doi:10.1073/pnas.2133846100. PMC 218772. PMID 14519844.
Sharma M, Pampinella F, Nemes C, et al. (2004). "Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes". J. Cell Biol. 164 (6): 923–33. doi:10.1083/jcb.200312018. PMC 2172283. PMID 15007060.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Bowers K, Piper SC, Edeling MA, et al. (2006). "Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII". J. Biol. Chem. 281 (8): 5094–105. doi:10.1074/jbc.M508632200. PMID 16371348.

This article incorporates text from the public domain Pfam and InterPro: IPR008570

This article on a gene on human chromosome 17 is a stub. You can help Wikipedia by adding missing information.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000131475 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000078656 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid15511219-5] Yorikawa C, Shibata H, Waguri S, Hatta K, Horii M, Katoh K, Kobayashi T, Uchiyama Y, Maki M (Mar 2005). "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting". Biochem J. 387 (Pt 1): 17–26. doi:10.1042/BJ20041227. PMC 1134928. PMID 15511219.

[entrez-6] "Entrez Gene: VPS25 vacuolar protein sorting 25 homolog (S. cerevisiae)".

[pmid17215868-7] Gill DJ, Teo H, Sun J, Perisic O, Veprintsev DB, Emr SD, Williams RL (January 2007). "Structural insight into the ESCRT-I/-II link and its role in MVB trafficking". EMBO J. 26 (2): 600–12. doi:10.1038/sj.emboj.7601501. PMC 1783442. PMID 17215868.

[pmid15469844-8] Teo H, Perisic O, Gonzalez B, Williams RL (October 2004). "ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes". Dev. Cell. 7 (4): 559–69. doi:10.1016/j.devcel.2004.09.003. PMID 15469844.

[pmid12194858-9] Babst M, Katzmann DJ, Snyder WB, Wendland B, Emr SD (August 2002). "Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body". Dev. Cell. 3 (2): 283–9. doi:10.1016/S1534-5807(02)00219-8. PMID 12194858.

[pmid15579210-10] Wernimont AK, Weissenhorn W (December 2004). "Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II". BMC Struct. Biol. 4 (1): 10. doi:10.1186/1472-6807-4-10. PMC 539351. PMID 15579210.

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VPS25

References

Further reading

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