Myles won a BAFTA for Best Actress for her role as Kate in Hallam Foe (2007) and was also nominated for a BIFA at the British Independent Film Awards for the same film. She has also won several other awards at various other film festivals across the world.
Since 1996, Myles has appeared in a number of films and television productions. Following her success in The Prince and the Pauper, Myles made her film debut in Mansfield Park (1999) and had a small role in Guest House Paradiso (1999). After roles in the television series Heartbeat and Foyle's War, Myles starred as Victoria Abberline in the thriller film From Hell (2001) alongside Johnny Depp, with Myles playing his wife. Myles played a main role in the comedy-romance film The Abduction Club (2002) before then playing the role of Erika in the 2003 vampire film Underworld with Kate Beckinsale, and reprised the character in its sequel, Underworld: Evolution (2006).
In 2003, she also starred in the thriller Out of Bounds and played Lady Penelope in Thunderbirds (2004), the film version of the classic television show of the same name. Myles initially had the choice to star in either Thunderbirds or Troy but she chose the former as she said "Lady Penelope is such an iconic character".[5] In 2005, Myles starred in the two-part war drama miniseries Colditz with Damian Lewis and Tom Hardy. In 2006, she co-starred as Isolde in the epic romantic drama Tristan & Isolde opposite James Franco and produced by Ridley Scott.
In 2007, Myles was cast as Beth Turner in the popular CBS supernatural television drama Moonlight, a role she would play until the show's end in 2008. Moonlight won Best New Drama in the 2007 People's Choice Awards. Myles was nominated for the Best Actress Award in 2007 for her role as Kate in Hallam Foe from the British Independent Film Award committee,[7] for which she also received a BAFTA Scotland Award. In 2008, she played Freya, the daughter of John Hurt's character, in the sci-fi action epic Outlander with Jim Caviezel and Ron Perlman.[8]
Later in 2014, Myles starred as Rachel in the psychological horror film Blackwood before going on to have roles as Dr. Anna Clarke in the international action-thriller Crossing Lines and the aristocratic Lady Katherine Longmore in the miniseries Our Zoo. In 2018, Myles provided her voice for an episode of the sci-fi series Firestorm.
Myles currently plays Rebecca Bishop in the fantasy-romance television series A Discovery of Witches; the first season was released in 2018 and the second in 2021 with a third series already filmed and awaiting release. After roles in the films Two Words (2018) and November 1st (2019), for which she won Best Actress and Best Supporting Actress respectively, Myles played Ann Payne, a secret agent, in the 2020 Portuguese film Listen which was selected as the Portuguese entry for the Best International Feature Film at the 93rd Academy Awards.[11] However, in December 2020 the film was disqualified, as more than half of its dialogue was in English.[12] Also in 2020, Myles reprised her role as Madame de Pompadour for a minisode sequel specially written for her by Steven Moffat that aired on YouTube on 6 May 2020.[13]
In 2021, Myles starred as Beth Barnes in the comedy-heist film Decrypted about the kidnap of the creator of Bitcoin, alongside Amanda Abbington and Kevin McNally.[14][15] In November 2021, she starred in the film All That Glitters playing a wealthy woman hiding the fact that she is domestically abused; a role that won her Best Actress at the New Renaissance Film Festival and the Gold Movie Awards respectively. In December 2021, Myles played Louise Campbell, Duchess of Argyll in the acclaimed BBC television mini-series A Very British Scandal with Claire Foy and Paul Bettany.
In September 2014, Myles gave birth to a son fathered by her partner James Bell, who works for the Bank of England.[21][22][23] Her son's godmother is Myles' friend and fellow actress Celia Imrie.[24]
^Sophia says her own name at the start of the video commentary for "The Girl in the Fireplace" in the DVD collection Doctor Who: The Complete Second Series.
^ abHusband, Stuart (5 August 2007). "Curtain call for Miss Myles". Guardian Unlimited. Archived from the original on 15 October 2007. Retrieved 5 August 2007.
^"2006 Final Nebula Award Ballot". sfwa.org. Science Fiction and Fantasy Writers of America, Inc. Archived from the original on 22 March 2007. Retrieved 12 April 2007.; "2007 Hugo Awards". thehugoawards.org. World Science Fiction Society. 1 September 2007. Archived from the original on 11 July 2013. Retrieved 1 September 2007.