Music source separation (MSS),[1] also known as stem separation, demixing, audio source separation or unmixing,[2] is a technique of separating
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Music source separation (MSS),[1] also known as stem separation, demixing, audio source separation or unmixing,[2] is a technique of separating one audio track into multiple audio tracks by targeting mixed material using music information retrieval (MIR).[3] MSS is a branch of signal separation which was established in the mid-1990s as a technology to reconstruct one or more source signals from mixtures of them. The process is generally utilized by music professionals to separate existing recordings for the purposes of enhancing the balance of the mix, remixing, or remastering. There are additional use cases where no multitrack or session files are available for the sound recording, making it necessary to rely on tools that provide stem separation from a single audio file.

Initial audio source separation for commercial purposes resulted in a file that was non-destructively separated, so that the resulting files could be reconstructed and sound exactly like the original without introducing issues when all tracks were performed simultaneously.[4]
There are a wide variety of applications of the technology outside of music including teaching, forensics, speech separation, live sound cancelation, audio restoration, and VR/AR.[5][6]
Starting late 2018, commercial tools became available for the separation of four part stems from a single audio file using AI models.[7] These applications separated stems into vocal, drums, bass, and other categories from a single audio file. Deep learning advancements and new processing models such as Wav-U-Net for neural networks aided in higher quality and fewer phase artifacts in separations from mixed material.[8] iZotope RX, AudiosourceRe Demix, RipX DeepMix were a handful of early providers of specialized stem separation tools. Eventually this process would become more widely adopted by users and commercial application developers; simultaneously, the technologies would continue to improve in terms of quality and speed of separation.[9] The company Deezer also made its Spleeter tool openly available around 2019 for further research and development into the audio source separation process.[10] Companies such as Apple would go on to release four part stem separation tools directly in the DAW (requiring Apple Silicon in this case), branded as Stem Splitter.[11] There are dozens of companies now utilizing the technologies involved with audio source separation to fit a multitude of applications. New separation options such as piano, strings, winds, guitar, acoustic guitar, and synthesizer are available from a variety of developers as of 2025. The trend for early 2025 was to include these stem separation capabilities with additional AI-based musically purposeful technologies such as mastering, pitch detection and manipulation, chord recognition, lyric transcription, vocal swapping and similar.
This process involving reverse engineering stems from mastered tracks relies on training models to identify targets in mixtures. Millions of real isolated stems from project files are used to update the parameter margins of models to generate estimates for the final output from mixtures. Large multitrack datasets are developed from the provided isolated stems with further adjustments to mixtures to provide higher numbers in the dataset that train the models for higher degrees of accuracy.[12] Initially, providers utilized online-based stem separation because it enabled the utilization of powerful computational systems (often involving advanced GPUs); now, there are many options for local system-based processing of the AI because of optimizations in the processing approach. There are also CPU developments that include neural workflows which facilitate the faster processing architecture needed for highest fidelity stem separation with lower time requirements.[13]
A growing number of companies are providing the ability for both music publishers and clients to utilize the stem separation technologies for their project needs, which is especially useful in the case of vocal removal from mixtures. Utilizing these tools provides editors and agents of the film and TV music industry to quickly have available the ability to adjust and contour songs without the need to reach out to providers which would cause time delays. This improves placement potential because a common issue with sync placements is that certain kinds of sounds (for example, vocals) can interfere too much with the underscore. This also provides the sync professionals the ability to take the track into unexpected directions and otherwise enhance the mix for the purpose of the application of the track.[14]
Quick stem separation is a perfect match for the professional DJ looking to create unique mashups. Generally, the track is rendered into a stem by placing the desired songs into the appropriate folder; when the song is selected, it has the basic four stem groupings available, and in some cases, individual parts (samples) can be triggered on pads for live performance.[15]
Disney Music Group used stem separation technologies to enhance their back catalog of recordings.[16] Beatles recordings were split and enhanced with stem separation technologies and the engineers during this process also helped to progress the development of the technology.[17] Numerous classic hit songs have been the target of restoration through stem separation achievements.[18][19]
Stems have been used in the recording industry to mean files bounced during the mixing process, generally a collection of like sounds grouped as a "stem". Stems in the context of the original project files can provide a large number of exported audio files for multiple purposes. These kinds of files generally provide a better quality overall and offer the ability to further isolate project material without introducing artifacts.
AI stem separations have generally produced material that is ideally suited for volume adjustments or further effect processing or production. These kinds of stems generally have come in the basic four groupings of vocal, bass, drum, and other. New approaches and deeper training of models resulted in the capability to isolate additional material beyond the basic four groupings, however these kinds of separations generally have spectral anomalies, blend in additional sounds, or change some quality of the original targeted sound.[20]
The process of using AI and other methodologies to target specific kinds of sounds happened to enable a new method of spectral separation-based sound design through new kinds of tools to edit with such as those in SpectraLayers and RipX. The instant ability to unmix components such as transient information and time-based information into full tracks of unconventional sound creations. Groove shadows and other sound production dubbing techniques are easily achievable by revealing new timbres and structures based on spectral selections because of the advancements into tools to support stem manipulation.[21][22]
Aside from advanced noise reduction methods based on learning noise profiles, taking an inverted approach and removing known source targets such as the basic four and specialized models can result in leaving only the noise as a separate track depending on the ensemble. From that, one can remove the noise track. Noise may result on only a single stem and that stem can be targeted exclusively with noise reduction profiles in this way the entire mix does not need to be processed.[23]
One of the most popular use cases of stem separation is for the purposes of creating an instrumental of a song where one isn't known to exist or available. There are dozens of sites using the technology to attract users aspiring to make such instrumental versions of their favorite songs.[24][25]
The RipX DAW is a unique take on the concept of stem separation because of its note-based harmonic audio visual structure branded as "Rip Audio Format". The system provides a stem separation tool that breaks down a single file into several tracks with notes being represented as the audio track. These notes are highly adjustable and the system includes highly specialized tools for working with the notes and the spectral aspects of the captures. Each note or note part can have specialized effects applied. Tracks can be swapped easily because of the utilization of this notation with other sounds entirely. Thus, the stem is not only separated but the MIDI is transcribed, making it possible to perform as a MIDI sequence and thereby direct instruments. The notation used by the Rip Audio format resembles the Melodyne architecture of note extraction from audio, these notes however also function as MIDI and audio simultaneously. RipX is a completely unique kind of DAW that is based around stem separation as well as this new Rip Audio format, where audio and midi worlds forge a symbiosis with new kinds of tools to support the new paradigm.[26]
Native Instruments created a specialized tool called "Stem Creator Tool" for working with four part stem tracks which is ideally suited for the DJ world as digital DJ consoles and Native Instruments hardware like Traktor and Maschine use the four track stem structure. This tool enables quick mastering and saving of files in a "stem" archival format.[27] The tool is free to use and essential mastering effects applicable to stem-based audio are provided.
The length of time it takes to analyze and separate the sound means that the mixtures generally need pre-rendering or there is a delay in processing. The process of AI-based stem separation produces artifacts and doesn't always result in the correct designation of target instruments. There may also be spectral bleed from part to part. It is easy to compromise the mixed structure of the full work by adjusting certain elements in isolated parts. A rapid volume-modulated sound similar to tremolo may also be a factor of certain kinds of separations. The order in which the source audio is processed and the kind of applications and their sequence can affect the outcome of separations in addition to the kind of mixes and masters. The process can pick up spectral anomalies which may need to be merged into different tracks. There may be a need to reprocess a stem separation of specialized instruments until the desired balance of the captured target sound is realized. Specialized audio editing tools exist to further clean up the processing of the stem separation.[28][29]
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