Lipopolysaccharide kinase (Kdo/WaaP) family

Kdo
Identifiers
Symbol	Kdo
Pfam	PF06293
Pfam clan	CL0016
InterPro	IPR010440
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

In molecular biology, the lipopolysaccharide kinase (Kdo/WaaP) family is a family of lipopolysaccharide kinases that includes lipopolysaccharide core heptose(I) kinase rfaP (encoded by the waaP (rfaP) gene). Lipopolysaccharide core heptose(I) kinase rfaP is required for the addition of phosphate to O-4 of the first heptose residue of the lipopolysaccharide (LPS) inner core region. It has previously been shown that it is necessary for resistance to hydrophobic and polycationic antimicrobials in E. coli and that it is required for virulence in invasive strains of Salmonella enterica.^[1] The family also includes 3-deoxy-D-manno-octulosonic acid kinase (KDO kinase) from Haemophilus influenzae, which phosphorylates Kdo-lipid IV(A), a lipopolysaccharide precursor, and is involved in virulence.^[2]

References

1. Yethon JA, Whitfield C (February 2001). "Purification and characterization of WaaP from Escherichia coli, a lipopolysaccharide kinase essential for outer membrane stability". J. Biol. Chem. 276 (8): 5498–504. doi:10.1074/jbc.M008255200. PMID 11069912.
2. White KA, Lin S, Cotter RJ, Raetz CR (1999). "A Haemophilus influenzae gene that encodes a membrane bound 3-deoxy-D-manno-octulosonic acid (Kdo) kinase. Possible involvement of kdo phosphorylation in bacterial virulence". J Biol Chem. 274 (44): 31391–400. doi:10.1074/jbc.274.44.31391. PMID 10531340.

Further reading

• Krupa A, Srinivasan N (2002). "Lipopolysaccharide phosphorylating enzymes encoded in the genomes of Gram-negative bacteria are related to the eukaryotic protein kinases". Protein Sci. 11 (6): 1580–4. doi:10.1110/ps.3560102. PMC 2373617. PMID 12021457.

This article incorporates text from the public domain Pfam and InterPro: IPR010440