Hydrolyzed vegetable protein

Hydrolyzed vegetable protein (HVP) products are foodstuffs obtained by the hydrolysis of protein, and have a meaty, savory taste similar to bouillon (broth).

Regarding the production process, a distinction can be made between acid-hydrolyzed vegetable protein (aHVP), enzymatically produced HVP, and other seasonings, e.g., fermented soy sauce. Hydrolyzed vegetable protein products are particularly used to round off the taste of soups, sauces, meat products, snacks, and other dishes, as well as for the production of ready-to-cook soups and bouillons.

History

Food technologists have long known that protein hydrolysis produces a meat bouillon-like odor and taste.^[1] Hydrolysates have been a part of the human diet for centuries, notably in the form of fermented soy sauce, or Shoyu. Shoyu, traditionally made from wheat and soy protein, has been produced in Japan for over 1,500 years, following its introduction from mainland China. The origins of producing these materials through the acid hydrolysis of protein (aHVP) can be traced back to the scarcity and economic challenges of obtaining meat extracts during the Napoleonic wars.^[2]

In 1831, Berzelius obtained products having a meat bouillon taste when hydrolysing proteins with hydrochloric acid.^[3] Julius Maggi produced acid-catalyzed hydrolyzed vegetable protein industrially for the first time in 1886.^[4]

In 1906, Fischer found that amino acids contributed to the specific taste.^[5] In 1954, D. Phillips found that the bouillon odor required the presence of proteins containing threonine.^[6] Another important substance that gives a characteristic taste is glutamic acid.

Manufacture

Almost all products rich in protein are suitable for the production of HVP. Today, it is made mainly from protein resources of vegetable origin, such as defatted oil seeds (soybean meal, grapeseed meal) and protein from maize (Corn gluten meal), wheat (gluten), pea, and rice.^[7] The process and the feedstock determines the organoleptic properties of the end product. Proteins consist of chains of amino acids joined through amide bonds. When subjected to hydrolysis (hydrolyzed), the protein is broken down into its component amino acids.

In aHVP, hydrochloric acid is used for hydrolysis. The remaining acid is then neutralized by mixing with an alkali such as sodium hydroxide, which leaves behind table salt, which comprises up to 20% of the final product (acid-hydrolyzed vegetable protein, aHVP).

In enzymatic HVP (eHVP), proteases are used to break down the proteins under a more neutral pH and lower temperatures.^[8] The amount of salt is greatly reduced.

Because of the different processing conditions, the two types of HVP have different sensory profiles. aHVP is usually dark-brown in color and has a strong savory flavor, whereas eHVP usually is lighter in color and has a mild savory flavor.^[9][10]

Acid hydrolysis

Acid hydrolysates are produced from various edible protein sources, with soy, corn, wheat, and casein being the most common. For the production of aHVP, the proteins are hydrolyzed by cooking with a diluted (15–20%) hydrochloric acid, at a temperature between 90 and 120 °C for up to 8 hours. After cooling, the hydrolysate is neutralized with either sodium carbonate or sodium hydroxide to a pH of 5 to 6. During hydrolysis, extraneous polymeric material known as humin, which forms from the interaction of carbohydrate and protein fragments, is generated and subsequently removed by filtration and then further refined.^[11]

The source of the raw material, concentration of the acid, the temperature of the reaction, the time of the reaction, and other factors can all affect the organoleptic properties of the final product. Activated carbon treatment can be employed to remove both flavor and color components, to the required specification. Following a final filtration, the aHVP may, depending upon the application, be fortified with additional flavoring components. Thereafter, the product can be stored as a liquid at 30–40% dry matter, or alternatively it may be spray dried or vacuum dried and further used as a food ingredient.^[11]

One hundred pounds (45kg) of material containing 60% protein will yield 100 pounds of aHVP, which contains approximately 40 pounds (18 kg) of salt. This salt gain occurs during the neutralization step.^[2]

Enzymatic hydrolysis

For the production process of enzymatic HVP, enzymes are used to break down the proteins. To break down the protein to amino acids, proteases are added to the mixture of defatted protein and water. Due to the sensitivity of enzymes to a specific pH, either an acid or a base is added to match the optimum pH. Depending on the activity of the enzymes, up to 24 hours are needed to break down the proteins. The mixture is heated to inactivate the enzymes and then filtered to remove the insoluble carbohydrates (humin).

Since no salt is formed during the production process, manufacturers may add salt to eHVP preparations to extend shelf life or to provide a product similar to conventional aHVP.

A commonly used protease mixture is "Flavourzyme", extracted from Aspergillus oryzae.^[12]

Composition

Liquid aHVP typically contains 55% water, 16% salt, 25% organic substances (thereof 20% protein (amino acids) analyzed as about 3% total nitrogen and 2% amino nitrogen).

Many amino acids have either a bitter or sweet taste. In many commercial processes, nonpolar amino acids such as L-leucine and L-isoleucine are often removed to create hydrolysates with a more mellow and less bitter character. D-tryptophan, D-histidine, D-phenylalanine, D-tyrosine, D-leucine, L-alanine, and glycine are known to be sweet, while bitterness is associated with L-tryptophan, L-phenylalanine, L-tyrosine, and L-leucine.^[2][13]

Tyrosine is an amino acid susceptible to halogenation during hydrolysis with HCl.^{[14][better source needed]} Lysine is stable under standard acid hydrolysis, but during heat treatment, the side-chain amino group can react with other compounds, such as reducing sugars, producing Maillard products.^{[15][better source needed]}

The organoleptic properties of HVP is determined not only by amino acid composition, but also by the various aroma-bearing substances other than the amino acids created during the production of both aHVP and eHVP. Aromas can be formed via amino acid decomposition, Maillard reaction, sugar cyclization, and lipid oxidation.^[16] A complex mix of aromas similar to butter, meat,^[17][16] bone stock,^[16] wood smoke,^[18] lovage^[19] and many other substances can be produced, depending on reaction conditions (time, temperature, hydrolysis method, additional feedstock such as xylose and spices).^[16][20]

According to the European Code of Practice for Bouillons and Consommés, hydrolyzed protein products intended for retail sale correspond to these characteristics:^[21]

• Specific gravity at 20°C min.: 1.22
• Total nitrogen min.: 4% (on dry matter)
• Amino nitrogen min.: 1.3% (on dry matter)
• Sodium chloride max.: 50% (on dry matter)

Use

When foods are produced by canning, freezing, or drying, some flavor loss is almost inevitable. Manufacturers can use HVP to make up for it.^[7] Therefore, HVP is used in a wide variety of products, such as in the spice, meat, fish, fine-food, snack, flavor, and soup industries.

Safety

3-MCPD

3-MCPD, a carcinogen in rodents and a suspected human carcinogen, is created during acid-hydrolysis as glycerol released from lipid (e.g. triglycerides) reacts with hydrochloric acid. Legal limits have been set to keep aHVP products safe for human consumption. aHVP manufacturers can reduce the amount of 3-MCPD to acceptable limits by (1) careful control of reaction time and temperature (2) timely neutralization of hydrochloric acid, optionally extending to an alkaline hydrolysis step to destroy any 3-MCPD already formed (3) replacement of hydrochloric acid with other acids such as sulfuric acid.^[11]

As an allergen

Whether hydrolyzed vegetable protein is an allergen or not is contentious.

According to European law, wheat and soy are subject to allergen labelling in terms of Regulation (EU) 1169/2011 on food information to consumers. Since wheat and soy used for the production of HVP are not exempted from allergen labelling for formal reasons, HVP produced by using those raw materials has to be labelled with a reference to wheat or soy in the list of ingredients.

Nevertheless, strong evidence indicates at least aHVP is not allergenic, since proteins are degraded to single amino acids which are not likely to trigger an allergic reaction. A 2010 study has shown that aHVP does not contain detectable traces of proteins or IgE-reactive peptides. This provides strong evidence that aHVP is very unlikely to trigger an allergic reaction to people who are intolerant or allergic to soy or wheat.^[22] Earlier peer-reviewed animal studies done in 2006 also indicate that soy-hypersensitive dogs do not react to soy hydrolysate, a proposed protein source for soy-sensitive dogs.^[23]

There are reports of a cosmetic-grade aHVP, Glupearl 19S (GP19S), inducing anaphylaxis when present in soap. Unlike food aHVP, this Japanese wheat aHVP is only very mildly hydrolyzed.^[24] The unusual chemical condition makes GP19S more allergenic than pure gluten.^[25] Newer regulations for cosmetic hydrolyzed wheat protein have been developed in response, requiring an average molecular mass of less than 3500 Da – about 35 residues long. In theory, "an allergen must have at least 2 IgE-binding epitopes, and each epitope must be at least 15 amino acid residues long, to trigger a type 1 hypersensitivity reaction." Experiments also show that this degree of hydrolysis is sufficient to not trigger IgE binding from GP19S-allergic patients.^[24]

Allergenicity of eHVP depends on the specific food source and the enzyme used. Alcalase is able to render chickpea and green pea completely non-immunoreactive but papain only achieves partial reduction. Alcalase is also unable to make white beans non-reactive due to the antinutritional factors preventing complete digestion.^[26] Alcalase, but not "Flavourzyme" (a commercial Aspergillus oryzae protease blend^[12] for eHVP production), is able to make roasted peanut non-reactive.^[27]

See also

• Hydrolyzed protein
• MSG

References

1. Manley/Fagerson (1971). Aspects of Aroma and Taste Characteristics of Hydrolysed Vegetable Protein, The Flavour Industry. p. 686 f.
2. Manley, C.H.; McCann, J.S.; Swaine, R.L. (1981), "The Chemical Bases of the Taste and Flavor Enhancing Properties of Hydrolyzed Protein", The Quality of Foods and Beverages, Elsevier, pp. 61–82, doi:10.1016/b978-0-12-169101-1.50011-2, ISBN 978-0-12-169101-1, retrieved May 15, 2024
3. "Würzen". Ullmanns Encyclopedia of Technical Chemistry. Vol. 18 (3rd ed.). Munich, Berlin, Vienna. 1967.
4. Reineccius (1994). Source Book of Flavours. Springer. p. 140.
5. Fischer, Research about amino acids, Springer 1906
6. D. M. P. Phillips (1954). "Threonine and the Odour of Protein Hydrolysates". Nature. 173 (4414): 1092–1093. Bibcode:1954Natur.173.1092P. doi:10.1038/1731092a0. S2CID 4246025.
7. Prendergast (1974). "Protein Hydrolysate – A review". Food Trade Review: 14 f.
8. Goesaert, Hans; Courtin, Christophe M.; Delcour, Jan A. (2008), "Use of enzymes in the production of cereal-based functional foods and food ingredients", Gluten-Free Cereal Products and Beverages, Elsevier, pp. 237–265, ISBN 978-0-12-373739-7, retrieved May 14, 2024
9. Aaslyng, Margit Dall; Martens, Magni; Poll, Leif; Nielsen, Per Munk; Flyge, Hanne; Larsen, Lone Melchior (February 1, 1998). "Chemical and Sensory Characterization of Hydrolyzed Vegetable Protein, a Savory Flavoring". Journal of Agricultural and Food Chemistry. 46 (2): 481–489. doi:10.1021/jf970556e. ISSN 0021-8561.
10. Weir, G. S. D. (1992), "Proteins as a Source of Flavour", Biochemistry of Food Proteins, Boston, MA: Springer US, pp. 363–408, ISBN 978-1-4684-9897-4, retrieved May 14, 2024
11. "Code of practice for the Reduction of 3-Monochlorpropanole-1,2-Diol (3-MCPD) during the Production of acid-HVPs and products that contain acid-HVPs CAC/RCP 64-2008" (PDF).
12. Merz, Michael; Eisele, Thomas; Berends, Pieter; Appel, Daniel; Rabe, Swen; Blank, Imre; Stressler, Timo; Fischer, Lutz (June 17, 2015). "Flavourzyme, an Enzyme Preparation with Industrial Relevance: Automated Nine-Step Purification and Partial Characterization of Eight Enzymes". Journal of Agricultural and Food Chemistry. 63 (23): 5682–5693. doi:10.1021/acs.jafc.5b01665. PMID 25996918.
13. Solms, Juerg (July 1969). "Taste of amino acids, peptides, and proteins". Journal of Agricultural and Food Chemistry (4): 686–688. doi:10.1021/jf60164a016. ISSN 0021-8561. Retrieved May 15, 2024.
14. Nissen, Steven (1992), "Amino Acid Analysis in Food and in Physiological Samples", Modern Methods in Protein Nutrition and Metabolism, Elsevier, pp. 1–8, ISBN 978-0-12-519570-6, retrieved May 15, 2024
15. Hurrell, R. F.; Carpenter, K. J. (1981). "The estimation of available lysine foodstuffs after Maillard reactions". Progress in Food & Nutrition Science. 5 (1–6): 159–176. ISSN 0306-0632. PMID 6798628.
16. Kale, Prajyoti; Mishra, Anusha; Annapure, Uday S. (June 2022). "Development of vegan meat flavour: A review on sources and techniques". Future Foods. 5: 100149. doi:10.1016/j.fufo.2022.100149. S2CID 248838839.
17. Wu, Yi-Fang G.; Cadwallader, Keith R. (May 8, 2002). "Characterization of the Aroma of a Meatlike Process Flavoring from Soybean-Based Enzyme-Hydrolyzed Vegetable Protein". Journal of Agricultural and Food Chemistry. 50 (10): 2900–2907. doi:10.1021/jf0114076. PMID 11982417.
18. Jarunrattanasri, Arporn; Theerakulkait, Chockchai; Cadwallader, Keith R. (April 1, 2007). "Aroma Components of Acid-Hydrolyzed Vegetable Protein Made by Partial Hydrolysis of Rice Bran Protein". Journal of Agricultural and Food Chemistry. 55 (8): 3044–3050. doi:10.1021/jf0631474. PMID 17367160.
19. Blank, Imre; Schieberle, Peter (July 1993). "Analysis of the seasoning-like flavour substances of a commercial lovage extract (Levisticum officinale Koch.)". Flavour and Fragrance Journal. 8 (4): 191–195. doi:10.1002/ffj.2730080405.
20. Li, Xuejie; Li, Jian (2020). "The Flavor of Plant-Based Meat Analogues". Cereal Foods World. 65 (4). doi:10.1094/CFW-65-4-0040. S2CID 231203281.
21. "Code of practice for Bouillons and Consommés".
22. Reuter A, Gerald R, Kuehne Y, Engin A, Przywara J, Worm M, Ballmer-Weber B, Holzhauser T, Vieths S. Evaluation of the allergenic potential of soy and wheat based seasonings. XXIX EAACI Congress of the European Academy of Allergy and Clinical Immunology. p. 322. doi:10.1111/j.1398-9995.2010.02393.x. PMC 7159487.
23. Puigdemont, A; Brazís, P; Serra, M; Fondati, A (March 2006). "Immunologic responses against hydrolyzed soy protein in dogs with experimentally induced soy hypersensitivity". American Journal of Veterinary Research. 67 (3): 484–8. doi:10.2460/ajvr.67.3.484. PMID 16506912.
24. Burnett, Christina; Bergfeld, Wilma F.; Belsito, Donald V.; Hill, Ronald A.; Klaassen, Curtis D.; Liebler, Daniel C.; Marks, James G.; Shank, Ronald C.; Slaga, Thomas J.; Snyder, Paul W.; Andersen, F. Alan; Heldreth, Bart (May 2018). "Safety Assessment of Hydrolyzed Wheat Protein and Hydrolyzed Wheat Gluten as Used in Cosmetics". International Journal of Toxicology. 37 (1_suppl): 55S–66S. doi:10.1177/1091581818776013. PMID 29761728. S2CID 46889452.
25. Nakamura, Masashi; Yagami, Akiko; Hara, Kazuhiro; Sano, Akiyo; Kobayashi, Tsukane; Matsunaga, Kayoko (February 2015). "Antigens in Glupearl 19S Were Developed By Acid-Heat Treatment". Journal of Allergy and Clinical Immunology. 135 (2): AB254. doi:10.1016/j.jaci.2014.12.1771.
26. Calcinai, Luisa; Bonomini, Maria Giulia; Leni, Giulia; Faccini, Andrea; Puxeddu, Ilaria; Giannini, Daiana; Petrelli, Fiorella; Prandi, Barbara; Sforza, Stefano; Tedeschi, Tullia (October 7, 2022). "Effectiveness of enzymatic hydrolysis for reducing the allergenic potential of legume by-products". Scientific Reports. 12 (1): 16902. Bibcode:2022NatSR..1216902C. doi:10.1038/s41598-022-21296-z. hdl:10807/230858. PMC 9547019. PMID 36207409.
27. Cabanillas, Beatriz; Pedrosa, Mercedes M.; Rodríguez, Julia; Muzquiz, Mercedes; Maleki, Soheila J.; Cuadrado, Carmen; Burbano, Carmen; Crespo, Jesús F. (2012). "Influence of Enzymatic Hydrolysis on the Allergenicity of Roasted Peanut Protein Extract". International Archives of Allergy and Immunology. 157 (1): 41–50. doi:10.1159/000324681. PMID 21912172. S2CID 25496670.