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Guillén or Guillen is a Spanish or French surname. Notable people with the surname include:

Alanis Guillen (born 1998), Brazilian actress.
Ambrosio Guillen (1929–1953), United States Marine who was posthumously awarded the Medal of Honor
Amparo Guillén (1953–2024), Ecuadorian actress
Carlos Guillén, retired Venezuelan baseball player
Charlie Guillen, American writer and filmmaker
Fernando Guillén (disambiguation), multiple people
Harvey Guillén (born 1990), actor
Jorge Guillén, Spanish poet
José Guillén (born 1976), Dominican baseball player
Juan Manuel Guillén, El Salvadoran artist, digital producer for LCBO
Laurice Guillén (born 1947), Filipina actress
Montserrat Guillén (born 1964), Spanish statistician and economist
Nancy Guillén (born 1976), retired Salvadoran hammer thrower
Néstor Guillén Olmos (1890–1966), President of Bolivia for 27 days
Nicolás Guillén, Afro-Cuban poet
Ozzie Guillén (born 1964), Venezuelan baseball coach and former player
Osiel Cárdenas Guillén (born 1967), Mexican drug lord
Rafael Guillen ("Subcomandante Marcos"), spokesman for the Zapatista Army of National Liberation
Ricardo Guillén (born 1967), Spanish basketball player
Sammy Guillen (1924–2013), West Indian and New Zealand cricket player
Tonatiuh Guillén López, Mexican academic and government official, Mexico's immigration chief since December 2018
Vanessa Guillen, American soldier
Walter Guillén Soto, Catholic bishop in Honduras

This page lists people with the surname Guillén.
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