G protein-gated ion channel

Generalized diagram of G protein-gated ion channel: (A) Typically, the activated effector protein begins a signaling cascade which leads to the eventual opening of the ion channel. (B) The GTP-bound α-subunit in some cases can directly activate the ion channel. (C) In other cases, the activated βγ-complex of the G protein may interact with the ion channel.

G protein-gated ion channels are a family of transmembrane ion channels in neurons and atrial myocytes that are directly gated by G proteins.

Overview of mechanisms and function

Generally, G protein-gated ion channels are specific ion channels located in the plasma membrane of cells that are directly activated by a family of associated proteins. Ion channels allow for the selective movement of certain ions across the plasma membrane in cells. More specifically, in nerve cells, along with ion transporters, they are responsible for maintaining the electrochemical gradient across the cell.

G proteins are a family of intracellular proteins capable of mediating signal transduction pathways. Each G protein is a heterotrimer of three subunits: α-, β-, and γ- subunits. The α-subunit (Gα) typically binds the G protein to a transmembrane receptor protein known as a G protein-coupled receptor, or GPCR. This receptor protein has a large, extracellular binding domain which will bind its respective ligands (e.g. neurotransmitters and hormones). Once the ligand is bound to its receptor, a conformational change occurs. This conformational change in the G protein allows Gα to bind GTP. This leads to yet another conformational change in the G protein, resulting in the separation of the βγ-complex (Gβγ) from Gα.[1] At this point, both Gα and Gβγ are active and able to continue the signal transduction pathway. Different classes of G protein-coupled receptors have many known functions including the cAMP and Phosphatidylinositol signal transduction pathways.[2] A class known as metabotropic glutamate receptors play a large role in indirect ion channel activation by G proteins. These pathways are activated by second messengers which initiate signal cascades involving various proteins which are important to the cell's response.

G protein-gated ion channels are associated with a specific type of G protein-coupled receptor. These ion channels are transmembrane ion channels with selectivity filters and a G protein binding site. The GPCRs associated with G protein-gated ion channels are not involved in signal transduction pathways. They only directly activate these ion channels using effector proteins or the G protein subunits themselves (see picture). Unlike most effectors, not all G protein-gated ion channels have their activity mediated by Gα of their corresponding G proteins. For instance, the opening of inwardly rectifying K+ (GIRK) channels is mediated by the binding of Gβγ.[3]

G protein-gated ion channels are primarily found in CNS neurons and atrial myocytes, and affect the flow of potassium (K+), calcium (Ca2+), sodium (Na+), and chloride (Cl) across the plasma membrane.[4]

Types of G Protein-gated ion channels

Potassium channels

Structure

Four G protein gated inwardly-rectifying potassium (GIRK) channel subunits have been identified in mammals: GIRK1, GIRK2, GIRK3, and GIRK4. The GIRK subunits come together to form GIRK ion channels. These ion channels, once activated, allow for the flow of potassium ions (K+) from the extracellular space surrounding the cell across the plasma membrane and into the cytoplasm. Each channel consists of domains which span the plasma membrane, forming the K+-selective pore region through which the K+ ions will flow.[5][6] Both the N-and C-terminal ends of the GIRK channels are located within the cytoplasm. These domains interact directly with the βγ-complex of the G protein, leading to activation of the K+ channel. .[7] These domains on the N-and C-terminal ends which interact with the G proteins contain certain residues which are critical for the proper activation of the GIRK channel. In GIRK4, the N-terminal residue is His-64 and the C-terminal residue is Leu-268; in GIRK1 they are His-57 and Leu-262, respectively. Mutations in these domains lead to the channel's desensitivity to the βγ-complex and therefore reduce the activation of the GIRK channel.[3]

The four GIRK subunits are 80-90% similar in their pore-forming and transmembrane domains, a feature accountable by the similarities in their structures and sequences. GIRK2, GIRK3, and GIRK4 share an overall identity of 62% with each other, while GIRK1 only shares 44% identity with the others.[6] Because of their similarity, the GIRK channel subunits can come together easily to form heteromultimers (a protein with two or more different polypeptide chains). GIRK1, GIRK2, and GIRK3 show abundant and overlapping distribution in the central nervous system (CNS) while GIRK1 and GIRK4 are found primarily in the heart.[4] GIRK1 combines with GIRK2 in the CNS and GIRK4 in the atrium to form heterotetramers; each final heterotetramer contains two GIRK1 subunits and two GIRK2 or GIRK4 subunits. GIRK2 subunits can also form homotetramers in the brain, while GIRK4 subunits can form homotetramers in the heart.[7] GIRK1 subunits have not been shown to be able to form functional homotetramers. Though GIRK3 subunits are found in the CNS, their role in forming functional ion channels is still unknown.[4]

Subtypes and respective functions

  • GIRKs found in the heart

One G protein-gated potassium channel is the inward-rectifing potassium channel (IKACh) found in cardiac muscle (specifically, the sinoatrial node and atria),[8] which contributes to the regulation of heart rate.[9] These channels are almost entirely dependent on G protein activation, making them unique when compared to other G protein-gated channels.[10] Activation of the IKACh channels begins with release of acetylcholine (ACh) from the vagus nerve[9] onto pacemaker cells in the heart.[10] ACh binds to the M2 muscarinic acetylcholine receptors, which interact with G proteins and promote the dissociation of the Gα subunit and Gβγ-complex.[11] IKACh is composed of two homologous GIRK channel subunits: GIRK1 and GIRK4. The Gβγ-complex binds directly and specifically to the IKACh channel through interactions with both the GIRK1 and GIRK4 subunits.[12] Once the ion channel is activated, K+ ions flow out of the cell and cause it to hyperpolarize.[13] In its hyperpolarized state, the neuron cannot fire action potentials as quickly, which slows the heartbeat.[14]

  • GIRKs found in the brain

The G protein inward rectifying K+ channel found in the CNS is a heterotetramer composed of GIRK1 and GIRK2 subunits[4] and is responsible for maintaining the resting membrane potential and excitability of the neuron.[9] Studies have shown the largest concentrations of the GIRK1 and GIRK2 subunits to be in the dendritic areas of neurons in the CNS.[4] These areas, which are both extrasynaptic (exterior to a synapse) and perisynaptic (near a synapse), correlate with the large concentration of GABAB receptors in the same areas. Once the GABAB receptors are activated by their ligands, they allow for the dissociation of the G protein into its individual α-subunit and βγ-complex so it can in turn activate the K+ channels. The G proteins couple the inward rectifying K+ channels to the GABAB receptors, mediating a significant part of the GABA postsynaptic inhibition.[4]

Furthermore, GIRKs have been found to play a role in a group of serotonergic neurons in the dorsal raphe nucleus, specifically those associated with the neuropeptide hormone orexin.[15] The 5-HT1A receptor, a serotonin receptor and type of GPCR, has been shown to be coupled directly with the α-subunit of a G protein, while the βγ-complex activates GIRK without use of a second messenger. The subsequent activation of the GIRK channel mediates hyperpolarization of orexin neurons, which regulate the release of many other neurotransmitters including noradrenaline and acetylcholine.[15]

Calcium channels

Structure

In addition to the subset of potassium channels that are directly gated by G proteins, G proteins can also directly gate certain calcium ion channels in neuronal cell membranes. Although membrane ion channels and protein phosphorylation are typically indirectly affected by G protein-coupled receptors via effector proteins (such as phospholipase C and adenylyl cyclase) and second messengers (such as inositol triphosphate, diacylglycerol and cyclic AMP), G proteins can short circuit the second-messenger pathway and gate the ion channels directly.[16] Such bypassing of the second-messenger pathways is observed in mammalian cardiac myocytes and associated sarcolemmal vesicles in which Ca2+ channels are able to survive and function in the absence of cAMP, ATP or protein kinase C when in the presence of the activated α-subunit of the G protein.[17] For example, Gα, which is stimulatory to adenylyl cyclase, acts on the Ca2+ channel directly as an effector. This short circuit is membrane-delimiting, allowing direct gating of calcium channels by G proteins to produce effects more quickly than the cAMP cascade could.[16] This direct gating has also been found in specific Ca2+ channels in the heart and skeletal muscle T tubules.[18]

Function

Several high-threshold, slowly inactivating calcium channels in neurons are regulated by G proteins.[13] The activation of α-subunits of G proteins has been shown to cause rapid closing of voltage-dependent Ca2+ channels, which causes difficulties in the firing of action potentials.[1] This inhibition of voltage-gated Calcium channels by G protein-coupled receptors has been demonstrated in the dorsal root ganglion of a chick among other cell lines.[13] Further studies have indicated roles for both Gα and Gβγ subunits in the inhibition of Ca2+ channels. The research geared to defining the involvement of each subunit, however, has not uncovered the specificity or mechanisms by which Ca2+ channels are regulated.

The acid-sensing ion channel ASIC1a is a specific G protein-gated Ca2+ channel. The upstream M1 muscarinic acetylcholine receptor binds to Gq-class G proteins. Blocking this channel with the agonist oxotremorine methiodide was shown to inhibit ASIC1a currents.[19] ASIC1a currents have also been shown to be inhibited in the presence of oxidizing agents and potentiated in the presence of reducing agents. A decrease and increase in acid-induced intracellular Ca2+ accumulation were found, respectively.[20]

Sodium channels

Patch clamp measurements suggest a direct role for Gα in the inhibition of fast Na+ current within cardiac cells.[21] Other studies have found evidence for a second-messenger pathway which may indirectly control these channels.[22] Whether G proteins indirectly or directly activate Na+ ion channels not been defined with complete certainty.

Chloride channels

Chloride channel activity in epithelial and cardiac cells has been found to be G protein-dependent. However, the cardiac channel that has been shown to be directly gated by the Gα subunit has not yet been identified. As with Na+ channel inhibition, second-messenger pathways cannot be discounted in Cl channel activation.[23]

Studies done on specific Cl channels show differing roles of G protein activation. It has been shown that G proteins directly activate one type of Cl channel in skeletal muscle.[10] Other studies, in CHO cells, have demonstrated a large conductance Cl channel to be activated differentially by CTX- and PTX-sensitive G proteins.[10] The role of G proteins in the activation of Cl channels is a complex area of research that is ongoing.

Clinical significance and ongoing research

Mutations in G proteins associated with G protein-gated ion channels have been shown to be involved in diseases such as epilepsy, muscular diseases, neurological diseases, and chronic pain, among others.[4]

Epilepsy, chronic pain, and addictive drugs such as cocaine, opioids, cannabinoids, and ethanol all affect neuronal excitability and heart rate. GIRK channels have been shown to be involved in seizure susceptibility, cocaine addiction, and increased tolerance for pain by opioids, cannabinoids, and ethanol.[24] This connection suggests that GIRK channel modulators may be useful therapeutic agents in the treatment of these conditions. GIRK channel inhibitors may serve to treat addictions to cocaine, opioids, cannabinoids, and ethanol while GIRK channel activators may serve to treat withdrawal symptoms.[24]

Alcohol intoxication

Alcohol intoxication has been shown to be directly connected to the actions of GIRK channels. GIRK channels have a hydrophobic pocket that is capable of binding ethanol, the type of alcohol found in alcoholic beverages.[25][26] When ethanol acts as an agonist, GIRK channels in the brain experience prolonged opening. This causes decreased neuronal activity, the result of which manifests as the symptoms of alcohol intoxication. The discovery of the hydrophobic pocket capable of binding ethanol is significant in the field of clinical pharmacology. Agents that can act as agonists to this binding site can be potentially useful in the creation of drugs for the treatment of neurological disorders such as epilepsy in which neuronal firing exceeds normal levels.[26]

Breast cancer

Studies have shown that a link exists between channels with GIRK1 subunits and the beta-adrenergic receptor pathway in breast cancer cells responsible for growth regulation of the cells. Approximately 40% of primary human breast cancer tissues have been found to carry the mRNA which codes for GIRK1 subunits.[27] Treatment of breast cancer tissue with alcohol has been shown to trigger increased growth of the cancer cells. The mechanism of this activity is still a subject of research.[27]

Down syndrome

Altered cardiac regulation is common in adults diagnosed with Down syndrome and may be related to G protein-gated ion channels. The KCNJ6 gene is located on chromosome 21 and encodes for the GIRK2 protein subunit of G protein-gated K+ channels.[28] People with Down Syndrome have three copies of chromosome 21, resulting in an overexpression of the GIRK2 subunit. Studies have found that recombinant mice overexpressing GIRK2 subunits show altered responses to drugs that activate G protein-gated K+ channels. These altered responses were limited to the sino-atrial node and atria, both areas which contain many G protein-gated K+ channels.[28] Such findings could potentially lead to the development of drugs that can help regulate the cardiac sympathetic-parasympathetic imbalance in Down Syndrome adults.

Chronic atrial fibrillation

Atrial fibrillation (abnormal heart rhythm) is associated with shorter action potential duration and believed to be affected by the G protein-gated K+ channel, IK,ACh.[29] The IK,ACh channel, when activated by G proteins, allows for the flow of K+ across the plasma membrane and out of the cell. This current hyperpolarizes the cell, thus terminating the action potential. It has been shown that in chronic atrial fibrillation there an increase in this inwardly rectifying current because of constantly activated IK,ACh channels.[29] Increase in the current results in shorter action potential duration experienced in chronic atrial fibrillation and leads to the subsequent fibrillating of the cardiac muscle. Blocking IK,ACh channel activity could be a therapeutic target in atrial fibrillation and is an area under study.

Pain management

GIRK channels have been demonstrated in vivo to be involved in opioid- and ethanol-induced analgesia.[30] These specific channels have been the target of recent studies dealing with genetic variance and sensitivity to opioid analgesics due to their role in opioid-induced analgesia. Several studies have shown that when opioids are prescribed to treat chronic pain, GIRK channels are activated by certain GPCRs, namely opioid receptors, which leads to the inhibition of nociceptive transmission, thus functioning in pain relief.[31] Furthermore, studies have shown that G proteins, specifically the Gi alpha subunit, directly activate GIRKs which were found to participate in propagation of morphine-induced analgesia in inflamed spines of mice.[32] Research pertaining to chronic pain management continues to be performed in this field.

See also

References

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King of Spain from 1746 to 1759 Ferdinand VIPortrait by Louis Michel Van Loo, c. 1746-59King of Spain (more...) Reign9 July 1746 – 10 August 1759PredecessorPhilip VSuccessorCharles IIIChief Ministers See list The Marquess of VillaríasJosé de CarvajalThe Duke of AlbaRicardo Wall Born23 September 1713Royal Alcazar of Madrid, Madrid, SpainDied10 August 1759(1759-08-10) (aged 45)Villaviciosa de Odón, Madrid, SpainBurialConvent of the Salesas RealesSpouse Barbara of Portugal ​...

 

Dynasty that ruled Kingdom of Gorkha (1559–1768) and Kingdom of Nepal (1768–2008) This article needs additional citations for verification. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed.Find sources: Shah dynasty – news · newspapers · books · scholar · JSTOR (April 2012) (Learn how and when to remove this template message) Shah Dynastyशाह वंशHouse of ShahRoyal ...

Pandemi COVID-19 di AljazairKasus terkonfirmasi menurut provinsi   1000+ kasus terkonfirmasi   500-999 kasus terkonfirmasi   300-499 kasus terkonfirmasi   100-299 kasus terkonfirmasi   1-99 kasus terkonfirmasiKematian terkonfirmasi menurut provinsi   100+ kematian   20-99 kematian   10-19 kematian   5-9 kematian   1-4 kematian   0 kematianPenyakitCOVID-19Galur virusSARS-CoV-2LokasiAlj...

 

Filmmaking in Malaysia Cinema of MalaysiaLFS Coliseum Cineplex, Kuala LumpurNo. of screens185,997 seats (2017) 1,094 screens (2017) 151 indoor cinemas (2017)[1][2] • Per capita183 seats per screen (2013) 3.5 screens per 100,000 inhabitants (2015) 4.6 indoor cinemas per 1,000,000 inhabitants (2013)[1]Main distributorsAstro ShawSkop ProductionsAnimonsta StudiosGSC Movies[3]Produced feature films (2019)[4][2]Total59Number of admiss...

 

У Вікіпедії є статті про інших людей із прізвищем Шалімов. Шалімов Олександр ОлексійовичШалимов Александр Алексеевич Народився 20 січня 1918(1918-01-20)с. Введенка, Задонський повіт, Воронезька губернія, РРФСРПомер 28 лютого 2006(2006-02-28) (88 років)Київ, Україна[1]Поховання Байков...

For the archaeological site, see Niya ruins. Town in Xinjiang, People's Republic of ChinaNiya نىيە尼雅TownMonument in downtown MinfengNiyaLocation in XinjiangCoordinates: 37°03′N 82°41′E / 37.050°N 82.683°E / 37.050; 82.683CountryPeople's Republic of ChinaRegionXinjiangPrefectureHotanCountyMinfeng/NiyaElevation1,405 m (4,610 ft)Population (2010)[1] • Total3,486Time zoneUTC+8 (China Standard)Postal code848500 Niya (Uyghur...

 

The Constitution (Forty-seventh Amendment) Act, 1984Parliament of India Long title An Act further to amend the Constitution of India. Citation47th AmendmentTerritorial extentIndiaPassed byLok SabhaPassed23 August 1984Passed byRajya SabhaPassed25 August 1984Assented to26 August 1984Commenced26 August 1984Legislative historyFirst chamber: Lok SabhaBill titleThe Constitution (Forty-eighth Amendment) Bill, 1983Introduced byHarinatha MisraIntroduced19 August 1983First reading19 Augu...

 

French director, actor, screenwriter and producer This biography of a living person needs additional citations for verification. Please help by adding reliable sources. Contentious material about living persons that is unsourced or poorly sourced must be removed immediately from the article and its talk page, especially if potentially libelous.Find sources: Pierre Jolivet – news · newspapers · books · scholar · JSTOR (January 2013) (Learn how and when ...

Transnistrian politician Nina ShtanskiFirst Lady of TransnistriaIn role19 September 2015 – 16 December 2016PresidentYevgeny ShevchukPreceded byVacantSucceeded bySvetlana KrasnoselskayaMinister of Foreign AffairsIn office24 January 2012 – 14 September 2015Prime MinisterPyotr StepanovTatiana TuranskayaMaija Parnas (Acting)Preceded byVladimir YastrebchakSucceeded byVitaly Ignatiev Personal detailsBorn (1977-04-10) 10 April 1977 (age 46)Tiraspol, Soviet Union(now Moldov...

 

Tantara RecordsParent companyBrigham Young UniversityFounded1993 (1993)FounderK. Newell DayleyGenreChoral, classical, contemporary, jazz, a capella, world musicCountry of originU.S.LocationProvo, UtahOfficial websitewww.tantararecords.com Tantara Records is a recording label owned by Brigham Young University (BYU) and operated by the BYU School of Music. The mission of Tantara is to promote the musical works of BYU, both by its various vocal and instrumental ensembles and also the works ...

 

Strategi Solo vs Squad di Free Fire: Cara Menang Mudah!