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Endo agar (also called Endo's medium) is a microbiological growth medium with a faint pink colour.^[1] Originally developed for the isolation of Salmonella typhi, it is now used mostly as a coliform medium. Most gram-negative organisms grow well in this medium, while growth of gram-positive organisms is inhibited.^[2] Coliform organisms ferment the lactose in this medium, producing a green metallic sheen (i.e. Escherichia coli), whereas non-lactose-fermenting organisms produce clear, colourless colonies,^[1] i.e. Salmonella species.

Typical composition

Endo agar typically contains (w/v):^[3]

1.0 % peptone
0.25 % dipotassium hydrogen phosphate (K₂HPO₄)
1.0 % lactose
0.33 % anhydrous sodium sulfite (Na₂SO₃)
0.03 % fuchsine
1.25 % agar

References

^ ^a ^b "Mondofacto". Archived from the original on March 3, 2016. Retrieved April 25, 2009.
^ "Traditional Microbiology". Becton, Dickinson and Co. (BD). 2006. Archived from the original on March 3, 2016. Retrieved November 28, 2022.
^ EMD Chemicals, "ENDO Agar". 2002. Archived from the original on April 13, 2009. Retrieved April 25, 2009.