Draft:TdTomato
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Submission declined on 9 May 2026 by ChrysGalley (talk). This draft provides insufficient context for those unfamiliar with the subject. Please see the guide to writing better articles for how to improve your writing.
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Comment: This is too summarised and provides insufficient context at this point. This needs a bit more explanation so that an intelligent reader can understand at least the lead section. Looking at the two sources, this material is available, even in the abstracts. As an encyclopedia we are a summary of reliable sources, and here it needs a bit more work. ChrysGalley (talk) 07:14, 9 May 2026 (UTC)
- Comment: In accordance with Wikipedia's Conflict of interest guideline, I disclose that I have a conflict of interest regarding the subject of this article. Maharjan.simran (talk) 22:10, 8 May 2026 (UTC)
tdTomato is a red fluorescent protein derived from DsRed through directed evolution and is characterized by low acid sensitivity. Originating from Discosoma species, tdTomato is reported to be six times brighter than enhanced green fluorescent protein (EGFP), with excitation and emission peaks at 554 nm and 581 nm, respectively..[1]. Despite being a tandem dimer, tdTomato functions as a monomer in fusion constructs. It is commonly used as an endoplasmic reticulum (ER) marker in various plant species for both transient and stable expression, and as a reporter gene to monitor gene expression, protein localization, and cell behavior.[2]
References
- ^ Chishti, A. A., Hellweg, C. E., Berger, T., Baumstark-Khan, C., Feles, S., Kätzel, T., & Reitz, G. (2015). Constitutive expression of tdTomato protein as a cytotoxicity and proliferation marker for space radiation biology. Life Sciences in Space Research, 4, 35–45. https://doi.org/10.1016/j.lssr.2014.12.005
- ^ Nagano, M., Ueda, H., Yoichiro Fukao, Maki Kawai-Yamada, & Ikuko Hara-Nishimura. (2020). Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochrome b5-B. Plant Signaling & Behavior, 15(9), 1790196–1790196. https://doi.org/10.1080/15592324.2020.1790196
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