Diaphonization

Diaphonization (or diaphonisation), also known as clearing and staining, is a staining technique used on animal specimens that first renders the body of the ani

Diaphonization
A diaphonized mirror dory. The bones are dyed red and the cartilage is dyed blue.
Diaphonized veiled chameleon. Field Museum of Natural History, Chicago.

Diaphonization (or diaphonisation), also known as clearing and staining, is a staining technique used on animal specimens that first renders the body of the animal transparent by bathing it in trypsin, and then stains the bones and cartilage with various dyes, usually alizarin red and alcian blue.[1]

History

Diaphonization was first developed by German anatomist Oskar Schultze in 1897, and later modified by numerous researchers.[2][3][4][5]

Technique

Clearing renders the animals transparent and is achieved by bathing the specimens in a soup of trypsin, a digestive enzyme that slowly breaks down flesh. The dyes alizarin red and alcian blue are most commonly used in the staining of bone and cartilage accordingly. When cleared, the specimen is put in glycerin. Despite its merits, diaphonization is not widely used in the scientific field. Advancements in imaging technology have rendered the practice all but obsolete, though it is expanding as an art form.[6]

Diaphonization is not suitable for animals longer than 30 centimeters (except for snakes) due to the limited ability of the trypsin bath to penetrate the tissues of larger animals. It is usually used to preserve animals that are too delicate to dissect, and instead are kept as wet specimens.[1]

References

  1. ^ a b Oommen, Ansel (2014-05-14). "Dyeing the Dead: The Artful Science of Diaphonization". Atlas Obscura. Retrieved 2021-02-05.
  2. ^ "Elaboration of transparent biological specimens for visualisation of developing cartilage and bone". ResearchGate. Retrieved 2021-02-05.
  3. ^ Dingerkus, Guido; Uhler, Lowell D. (1977). "Enzyme Clearing of Alcian Blue Stained Whole Small Vertebrates for Demonstration of Cartilage". Stain Technology. 52 (4): 229–232. doi:10.3109/10520297709116780.
  4. ^ Dwyer, Joseph; Ramirez, M. Desmond; Katz, Paul S.; Karlstrom, Rolf O.; Bergan, Joseph (2021). "Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force". Scientific Reports. 11 (1). The National Center for Biotechnology Information: 16462. Bibcode:2021NatSR..1116462D. doi:10.1038/s41598-021-95692-2. PMC 8360944. PMID 34385489.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  5. ^ Green, Margaret C. "A RAPID METHOD FOR CLEARING AND STAINING SPECIMENS FOR THE DEMONSTRATION OF BONE" (PDF). Knowledge Bank. The Ohio State University.
  6. ^ "The Artful Science of Diaphonization". 20 July 2015.
Wikimedia Commons logo
Wikimedia Commons has media related to Cleared and stained specimens.

Content Disclaimer

Informasi ini disarikan dari Wikipedia dan disajikan kembali untuk tujuan edukasi. Konten tersedia di bawah lisensi CC BY-SA 3.0. Kami tidak bertanggung jawab atas ketidakakuratan data yang bersumber dari kontribusi publik tersebut.

  1. The information displayed on this website is sourced in part or in whole from Wikipedia and has been adapted for the purpose of restating it. We strive to provide accurate and relevant information, however:
  2. There is no guarantee of absolute accuracy. Wikipedia is an open, collaborative project that can be edited by anyone, so information is subject to change.
  3. It is not intended to constitute professional advice. The content displayed is for informational and educational purposes only. For important decisions (e.g., medical, legal, or financial), please consult a professional.
  4. Content copyright. Wikipedia is licensed under the Creative Commons Attribution-ShareAlike License (CC BY-SA). This means that content may be reused with appropriate attribution and shared under a similar license.
  5. Responsible use. Any risk arising from the use of information from this website is entirely the responsibility of the user.