The 2016–17 Tweede Divisie season was the first edition of the Dutch third tier since its initial dissolution from the 1970-71 season and the fifteenth overall using "Tweede Divisie" name. Fourteen teams were completed from the TopklasseSaturday and Sunday divisions. Seven highest-placed teams of each division earned promotion to constitute a new, amateur Tweede Divisie. This change in the league system was approved in a KNVB assembly in December 2014. Thus, the Topklasse and leagues below it decremented by one level, and furthermore, promotion and relegation between the Tweede Divisie and the Topklasse were implemented.[1][2]
^AFC were deducted 1 point for not having the required number of players with a contract.[3]
^Jong Vitesse were deducted three points for having 3 players from the first team on the field at the same time, while the rules only allow 2 players from the first team on the field at the same time.[4]