PROFILBARU.COM

In pathology, silver staining is the use of silver to selectively alter the appearance of a target in microscopy of histological sections; in temperature gradient gel electrophoresis; and in polyacrylamide gels.

In traditional stained glass, silver stain is a technique to produce yellow to orange or brown shades (or green on a blue glass base), by adding a mixture containing silver compounds (notably silver nitrate), and firing lightly. It was introduced soon after 1800, and is the "stain" in the term "stained glass". Silver compounds^[1] are mixed with binding substances, applied to the surface of glass, and then fired in a furnace or kiln.^[2]^[3]^[4]

History

Camillo Golgi perfected silver staining for the study of the nervous system. Although the exact chemical mechanism by which this occurs is unknown,^[5] Golgi's method stains a limited number of cells at random in their entirety.^[6]

Silver staining was introduced by Kerenyi and Gallyas as a sensitive procedure to detect trace amounts of proteins in gels.^[7] The technique has been extended to the study of other biological macromolecules that have been separated in a variety of supports.^[8]

Classical Coomassie brilliant blue staining can usually detect a 50 ng protein band; silver staining increases the sensitivity typically 50 times.

Many variables can influence the color intensity and every protein has its own staining characteristics; clean glassware, pure reagents, and water of highest purity are the key points to successful staining.^[9]

Chemistry

Some cells are argentaffin. These reduce silver solution to metallic silver after formalin fixation. Other cells are argyrophilic. These reduce silver solution to metallic silver after being exposed to the stain that contains a reductant, for example hydroquinone or formalin.

Silver nitrate forms insoluble silver phosphate with phosphate ions; this method is known as the Von Kossa Stain. When subjected to a reducing agent, usually hydroquinone, it forms black elementary silver. This is used for study of formation of calcium phosphate particles during bone growth.

Applications

Histological characterisation

Silver staining aids the visualization of targets of interest, namely intracellular and extracellular cellular components such as DNA and proteins, such as type III collagen and reticulin fibres by the deposition of metallic silver particles on the targets of interest.^[10]

Diagnostic microbiology

Pseudomonas,^[11] Legionella, Leptospira, H. pylori, Bartonella and Treponema, and fungi such as Pneumocystis, Cryptococcus, and Candida are organisms that are stained with silver.^{[citation needed]}

Karyotype analysis

Silver staining is used in karyotyping. Silver nitrate stains the nucleolar organization region (NOR)-associated protein, producing a dark region wherein the silver is deposited and denoting the activity of rRNA genes within the NOR. Human chromosomes 13, 14, 15, 21, and 22 have NORs, which increase the silver stain activity by at least 50 times.^{[citation needed]}

Genomic and proteomic analysis

Silver staining is used to stain gels. The silver stain of proteins in Agarose gels was developed in 1973 by Kerenyi and Gallyas.^[12] Later it was adapted to polyacrylamide gels used in SDS-PAGE,^[13]^[14]^[15]^[16]^[17] and also for staining DNA or RNA.^[18] The glycosylations of glycoproteins and polysaccharides can be oxidised by a 1-hour pre-treatment with 0.1% periodic acid at 4 °C, which improves the binding of silver ions and the staining result.^[19]

First, the proteins are denatured in the gel by a fixative solution of 10% acetic acid and 30% ethanol and precipitated, at the same time the detergent (mostly SDS) is extracted. The diffusion of the proteins is thus significantly reduced. After repeated washing with water, the gel is incubated in a silver nitrate solution. Silver ions bind to negatively charged side chains of the proteins. Excess silver ions are then washed off with water. In the final development step, the silver ions are reduced to elemental silver by addition of alkaline formaldehyde. This stains the sites where proteins are present, brown to black.

The intensity of the staining depends on the primary structure of the protein. Furthermore, the cleanliness of the vessels used and the purity of the reagents influence the silver stain.^[20] Common artifacts in silver stained gels are bands of keratin in the ranges of 54–57 kDa and 65–68 kDa^[21] as a contamination of the sample prior to the electrophoresis.

Methenamine silver stains

There are several silver stains incorporating methenamine, including:

Grocott's methenamine silver stain, used widely as a screen for fungal organisms.
Jones' stain, a methenamine silver–periodic acid–Schiff that stains for basement membrane, availing to view the "spiked" GBM associated with membranous glomerulonephritis.

Gallery

A silver stain (GMS) demonstrating the fungus Histoplasma (black round balls) in a liver biopsy.
DNA samples amplified using PCR. Samples have been visualized using silver staining.
RBC membrane proteins separated by SDS-PAGE and silver-stained.^[22]

References

^ Steinhoff, Frederick Louis (1973). Ceramic Industry. Industrial Publications, Incorporated.
^ Chambers's encyclopaedia. Pergamon Press. 1967.
^ "Facts about Glass: Silver Stain". Boppard Conservation Project – Glasgow Museums. 18 July 2013.
^ Historic England (2011). Glass and glazing. Practical Building Conservation. Ashgate Publishing, Ltd. p. 290. ISBN 978-0754645573.
^ Golgi C (1873). "Sulla struttura della sostanza grigia del cervello". Gazzetta Medica Italiana (Lombardia). 33: 244–246.
^ Grant G (Oct 2007). "How the 1906 Nobel Prize in Physiology or Medicine was shared between Golgi and Cajal". Brain Res Rev. 55 (2): 490–498. doi:10.1016/j.brainresrev.2006.11.004. PMID 17306375. S2CID 24331507.
^ Kerenyi L, Gallyas F (1973). "Über Probleme der quantitiven Auswertung der mit physikalischer Entwicklung versilberten Agarelektrophoretogramme". Clin. Chim. Acta. 47 (3): 425–436. doi:10.1016/0009-8981(73)90276-3. PMID 4744834.
^ Switzer RC 3rd, Merril CR, Shifrin S (Sep 1979). "A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels". Anal. Biochem. 98 (1): 231–237. doi:10.1016/0003-2697(79)90732-2. PMID 94518.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: numeric names: authors list (link)
^ Hempelmann E, Schulze M, Götze O (1984). "Free SH-groups are important for the polychromatic staining of proteins with silver nitrate". Neuhof V (Ed)Electrophoresis '84, Verlag Chemie Weinheim 1984: 328–330.
^ Schwint OA, Labraga M, Cervino CO, et al. (2004). "A modification of the staining technique of reticular fibres for image analysis of the cardiac collagen network". Cardiovasc. Pathol. 13 (4): 213–20. doi:10.1016/S1054-8807(03)00153-4. PMID 15210137.
^ Barnini S, Dodi C, Campa M (2004). "Enhanced resolution of random amplified polymorphic DNA genotyping of Pseudomonas aeruginosa". Lett. Appl. Microbiol. 39 (3): 274–7. doi:10.1111/j.1472-765X.2004.01576.x. PMID 15287874.
^ Kerényi L, Gallyas F (September 1973). "[Errors in quantitative estimations on agar electrophoresis using silver stain]". Clin. Chim. Acta. 47 (3): 425–36. doi:10.1016/0009-8981(73)90276-3. PMID 4744834.
^ Merril CR, Switzer RC, Van Keuren ML (1979). "Trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain". Proc Natl Acad Sci U S A. 76 (9): 4335–9. Bibcode:1979PNAS...76.4335M. doi:10.1073/pnas.76.9.4335. PMC 411569. PMID 92027.
^ R. C. Switzer, C. R. Merril, S. Shifrin (September 1979), "A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels", Anal Biochem (in German), vol. 98, no. 1, pp. 231–237, doi:10.1016/0003-2697(79)90732-2, PMID 94518{{citation}}: CS1 maint: multiple names: authors list (link)
^ Rabilloud T, et al. (1988). "Improvement and simplification of low-background silver staining of proteins by using sodium dithionite". Electrophoresis. 9 (6): 288–291. doi:10.1002/elps.1150090608. PMID 2466660. S2CID 33007991.
^ Rabilloud T (1992). "A comparison between low background silver diammine and silver nitrate protein stains". Electrophoresis. 13 (7): 429–439. doi:10.1002/elps.1150130190. PMID 1425556. S2CID 43084621.
^ Lelong C, Chevallet M, Luche S, Rabilloud T (2009). "Silver Staining of Proteins in 2DE Gels". Two-Dimensional Electrophoresis Protocols (PDF). Methods Mol Biol. Vol. 519. pp. 339–350. doi:10.1007/978-1-59745-281-6_21. ISBN 978-1-58829-937-6. PMID 19381593. S2CID 52820065.
^ Blum H, Beier H, Gross HJ (1987). "Improved silver staining of plant protein, RNA & DNA in PAA gels". Electrophoresis. 8: 93–99. doi:10.1002/elps.1150080203. S2CID 84471792.
^ Dubray G, Bezard G (1982). "A highly sensitive periodic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gels". Anal. Biochem. 119 (2): 325–329. doi:10.1016/0003-2697(82)90593-0. PMID 6176144.
^ E. Hempelmann, M. Schulze, O. Götze: Free SH-groups are important for the polychromatic staining of proteins with silver nitrate. In: V. Neuhof (Editor): Electrophoresis. Verlag Chemie, Weinheim, 1984, pp. 328–330.
^ Ochs D (1983). "Protein contaminants of sodium dodecyl sulfate-polyacrylamide gels". Anal Biochem. 135 (2): 470–4. doi:10.1016/0003-2697(83)90714-5. PMID 6197906.
^ Hempelmann E, Götze O (1984). "Characterization of membrane proteins by polychromatic silver staining". Hoppe-Seyler's Z Physiol Chem. 365: 241–242.

External links

MedEd at Loyola Histo/practical/stains/hp2-55.html
[1] Hempelmann E. SDS-Protein PAGE and protein detection by silverstaining and immunoblotting of Plasmodium falciparum proteins. in: Moll K, Ljungström J, Perlmann H, Scherf A, Wahlgren M (eds) Methods in Malaria Research, 5th edition, 2008, 263-266