The song was later reprised as an instrumental version in another quintessential noir film, 1953's The Big Heat, when Gilda star Glenn Ford first meets Lee Marvin's character in a bar. It is again played in 1954's Human Desire, also starring Glenn Ford. But even in 1946, the year it was released, the song was employed in the B-movie Columbia release The Secret of the Whistler when the protagonist (played by series lead Richard Dix) meets the femme fatale.
The song is featured in the 2024 DC Studios miniseries The Penguin, a spin-off of the 2022 film The Batman. A clip of Hayworth's performance of the song is featured in the first episode, "After Hours", and the song is used again in the sixth episode, "Gold Summit", with protagonist Oz Cobb (Colin Farrell) comparing his elderly, dementia-ridden mother to Gilda.
In 2004, "Put the Blame on Mame" finished #84 in AFI's 100 Years...100 Songs survey of the top tunes in American cinema.