Pseudo-nitzschia australis

Pseudo-nitzschia australis
Scientific classification
Domain:	Eukaryota
Clade:	Diaphoretickes
Clade:	SAR
Clade:	Stramenopiles
Phylum:	Gyrista
Subphylum:	Ochrophytina
Class:	Bacillariophyceae
Order:	Bacillariales
Family:	Bacillariaceae
Genus:	Pseudo-nitzschia
Species:	P. australis
Binomial name
Pseudo-nitzschia australis Frenguelli, 1939

Pseudo-nitzschia australis is a pennate diatom found in temperate and sub-tropic marine waters, such as off the coast of California and Argentina. This diatom is a Harmful Micro Algae^[1] that produces toxic effects on a variety of organisms through its production of domoic acid, a neurotoxin. Toxic effects have been observed in a variety of predatory organisms such as pelicans, sea lions, and humans. If exposed to a high enough dose, these predators will die as a result, and there is no known antidote. The potential indirect mortality associated with P. australis is of great concern to humans as toxic algae blooms, including blooms of P. australis, continue to increase in frequency and severity over recent years. Blooms of P. australis are believed to result from high concentrations of nitrates and phosphates in stream and river runoff, as well as coastal upwelling, which are also sources of other harmful algae blooms.^[2]

Morphology

Image of Pseudo-nitzschia

Pseudo-nitzschia australis are a part of the genus which are bilaterally symmetrical diatoms with a protective cell wall layer called a silica.^[3] Their body plan is such that cells overlap with adjacent cells, allowing them to form chains. The cells are needle shaped, and the overlap between cells can be between one-third to one-half each cell length. Their total body length can be from 68-144 μm long and their width can range from 3-8 μm wide.^[2]

Life cycle

Pseudo-nitzschia australis can reproduce both sexually and asexually by binary fission.^[4] Sexual reproduction occurs by auxo-sporulation in which gametes fuse to form a zygote. Sexual reproduction has also been found to correlate with higher levels of domoic acid production^[5] Population growth of this species is seasonal and can depend on the amount of water upwelled and nutrient concentrations present off of the coast.^[4]

Habitat

These diatoms in a neritic zone meaning that they reside in shallow parts of the ocean with a depth ranging from 0 to 170 meters deep. Their temperature range is from around -1 °C to 29 °C.^[2] They can live in temperate and subtropical waters. Common distributions are the American West Coast, Australia, New Zealand, and North Atlantic Ocean.^[6]

Harmful algae blooms

There are many sources of harmful algae blooms. For instance, coastal upwellings can bring harmful algae to surface waters where they can photosynthesize due to an increase in light at the surface waters. This increase in photosynthesis can increase algal reproduction and the release of domoic acid.^[7] In addition to natural events, human activities such as deforestation and farming can lead to an increase in nutrients in nearby watersheds. As nutrient levels in the water increase, the algae population can increase to excessive levels. Blooms off of the Pacific Coast and Gulf of Mexico have increased in frequency over the 21st century, which scientists speculate may be due to global climate change.^[8] Algae blooms are considered harmful when the algae produce toxins that cause a deleterious effect on the ecosystem. P. australis produces domoic acid, which is what makes them dangerous to other organisms.^[2]

Effects on humans

Amnesic shellfish poisoning

Domoic acid can cause Amnesic Shellfish Poisoning (ASP) in humans that eat them. ASP is only a concern when algae in the ecosystem increases to high levels during an algal bloom. Shellfish are susceptible to accumulation of toxins due to their morphology as filter feeders. When they filter feed algae containing domoic acid, they can have accumulation of this toxin in their tissue. The poison has been detected in organisms such as mussels, oysters and clams and crabs. Physical symptoms in humans from ASP include diarrhea, nausea, vomiting, and abdominal cramps. Neurological symptoms can include headaches, disorientation, dizziness, short-term memory loss, coma and death.^[9] Cooking organisms which have accumulated domoic acid will not reduce their toxicity.^[10]

Economics

Peudo-nitzschia australius algal blooms can have a negative effect on fisheries and local economies. Due to amnesic shellfish poisoning (ASP) and its major threat to human health, fisheries are required to close when domoic acid levels reach an unsafe level in order to protect human safety.^[11] In New Zealand, one fishery had to shut down for four years while the domoic acid levels were considered too high to be safe.^[12]

Notable Pseudo-nitzschia australis blooms

Monterey Bay

Monterey Bay, located in central California, experienced a toxic P. australis algal bloom in the spring of 2015 leading to detrimental ecological issues such as marine organism illness and mortality. This bloom was caused by an unusual prolonged period of oceanic warming and upwelling which created conditions that allowed for an explosion of the P. australis species. The increase of available nitrogen from upwelling allowed for an increase in domoic acid synthesis. This bloom event lead to the temporary closure of fisheries such as razor clam and crab fisheries up the west coast from California to Washington due to the harmful impacts of domoic acid on human health. The closure of fisheries led to a request from the governor of California for a federal disaster declaration^[11] Effects of this harmful algal bloom on wildlife included mortality and seizures of a wide variety of marine species off of the coast.^[13] Domoic acid was detected in marine mammals such as whales, dolphins, sea lions, seals, and porpoises.^[11]

French Atlantic Coast

The first noted occurrence of the unusual toxic bloom of pseudo-nitzschia species on the French Atlantic coast happened in 1995. Pseudo-nitschia species have been observed in the waters every spring and autumn since 2006, but in low quantities. 2010 was an outlier year in which there was a larger bloom most likely caused by upwelling and nutrient runoff from a large storm. Additionally, this was the first year the species P. australis had been observed. This bloom resulted in a ban on shellfish harvesting due to toxic domoic acid build up in bivalves off of the coast.^[14]

Puget Sound

The P. australis was first observed in Washington in razor clams in 1991. The levels of domoic acid were above USDFA standards from 1991 to 2003 on the coast of Washington State. The toxin causes amnesic shellfish poisoning (ASP) and so the coast was closed for shellfishing while a domoic acid monitoring program was established. After deaths from ASP occurred in 1940, there have been monitoring programs in Washington state to monitor domoic acid levels in marine organisms known for accumulating domoic acid.^[15]

Toxicity

Domoic acid (DA) is a neurotoxin known to cause Amnesic Shellfish Poisoning (ASP) in humans and other predators that consume contaminated aquatic organisms that have consumed P. australius.^[16] DA has been isolated in several species of red algae, but is produced mostly by members of the Pseudo-nitzschia genus.

Chemical structure of domoic acid

The neurotoxicity of DA is due to its classification as a glutamate receptor agonist. An agonist causes an increase in cellular function, so it prolong neurological signals after the signal should have ceased to exist. In the case of DA, glutamate receptors are activated to open ion-gated channels in neurons, which leads to a variety of neurotoxic effects.^[17] At high enough doses, an organism will die of DA poisoning, while sublethal levels may cause a diverse range of effects on the central nervous system. There have been many experimental studies performed on animals, in addition to analysis of animals that were poisoned by DA in nature. Experiments showed that mammals exhibit a variety of behavioral and motor impairments, such as seizing, scratching, twitching, yawning, and head-waving. Upon dissection, organisms exposed acutely and chronically to DA were found to have brain lesions along the hippocampus and limbic system. Additionally, gross cardiac lesions were found in California sea lions, and retinal lesions found in some but not all species examined.^[18] DA exhibits both acute and chronic toxicity, and has no known antidote.^[19]

Effects on marine life

Marine mammals and seabirds have displayed adverse effects when in contact with Domoic acid including neurological effects and mortality.^[20] However, organisms such as shellfish and fish can ingest and accumulate domoic acid without ill effects.^[21] Despite these organisms not being effected, they can contribute to toxicity accumulating in the organisms that eat them. Domoic acid can accumulate up the food chain and cause adverse effects in marine organisms that are in higher trophic levels. It can cause neurological syndromes, gastrointestinal syndromes, acute retina issues, motor sensory abnormalities, heart defects, and morphological brain changes.^[22]

References

1. "IOC-UNESCO Taxonomic Reference List of Harmful Micro Algae (HABs)". www.marinespecies.org. Retrieved 2019-06-05.
2. "EOS - Phytoplankton Encyclopedia Project". www.eoas.ubc.ca. Retrieved 2019-06-05.
3. Kuwata, Akira; Jewson, David H. (2015), Ohtsuka, Susumu; Suzaki, Toshinobu; Horiguchi, Takeo; Suzuki, Noritoshi (eds.), "Ecology and Evolution of Marine Diatoms and Parmales", Marine Protists: Diversity and Dynamics, Springer Japan, pp. 251–275, doi:10.1007/978-4-431-55130-0_10, ISBN 9784431551300
4. Figueroa, Rosa Isabel; Estrada, Marta; Garcés, Esther (2018). "Life histories of microalgal species causing harmful blooms: Haploids, diploids and the relevance of benthic stages". Harmful Algae. 73: 44–57. doi:10.1016/j.hal.2018.01.006. ISSN 1568-9883. PMID 29602506.
5. Peterson, Jay; Hunter, Matt; Fisher, Jennifer; Peterson, William; Du, Xiuning (2016-10-12). "Initiation and Development of a Toxic and Persistent Pseudo-nitzschia Bloom off the Oregon Coast in Spring/Summer 2015". PLOS ONE. 11 (10): e0163977. Bibcode:2016PLoSO..1163977D. doi:10.1371/journal.pone.0163977. ISSN 1932-6203. PMC 5061394. PMID 27732630.
6. "WoRMS - World Register of Marine Species - Pseudo-nitzschia australis Frenguelli, 1939". www.marinespecies.org. Retrieved 2019-06-05.
7. Haschek, Wanda M.; Rousseaux, Colin G.; Wallig, Matthew A., eds. (2013). Haschek and Rousseaux's Handbook of Toxicologic Pathology. Elsevier. doi:10.1016/c2010-1-67850-9. ISBN 978-0-12-415759-0.
8. US EPA, OW (2013-09-05). "Climate Change and Harmful Algal Blooms". US EPA. Retrieved 2019-06-05.
9. "Amnesic Shellfish Poisoning (ASP) :: Washington State Department of Health". www.doh.wa.gov. Retrieved 2019-06-07.
10. "Harmful Algal Blooms Threaten Public Health and Economic Stability Along the West Coast". Science in the News. 2015-09-19. Retrieved 2019-06-08.
11. Ryan, J. P.; Kudela, R. M.; Birch, J. M.; Blum, M.; Bowers, H. A.; Chavez, F. P.; Doucette, G. J.; Hayashi, K.; Marin, R. (2017-06-05). "Causality of an extreme harmful algal bloom in Monterey Bay, California, during the 2014-2016 northeast Pacific warm anomaly". Geophysical Research Letters. 44 (11): 5571–5579. Bibcode:2017GeoRL..44.5571R. doi:10.1002/2017gl072637. ISSN 0094-8276.
12. Rhodes, Lesley; Scholin, Chris; Garthwaite, Ian (1998–2005). "Pseudo-nitzschia in New Zealand and the role of DNA probes and immunoassays in refining marine biotoxin monitoring programmes". Natural Toxins. 6 (3–4): 105–111. doi:10.1002/(sici)1522-7189(199805/08)6:3/4<105::aid-nt13>3.0.co;2-9. ISSN 1056-9014. PMID 10223626.
13. Fulton-Bennett, Kim (2017-06-05). "What caused the most toxic algal bloom ever observed in Monterey Bay?". MBARI. Retrieved 2019-06-05.
14. Nezan, Elisabeth Chomerat, Nicolas Bilien, Gwenael Boulben, Sylviane Duval, Audrey Ryckaert, Mireille (2010). Pseudo-nitzschia australis on French Atlantic coast - an unusual toxic bloom. IOC of Unesco. OCLC 1083122397.
15. "Harmful algal blooms in Puget Sound | Encyclopedia of Puget Sound". www.eopugetsound.org. Retrieved 2019-06-07.
16. Álvarez, Gonzalo; Uribe, Eduardo; Quijano-Scheggia, Sonia; López-Rivera, Américo; Mariño, Carmen; Blanco, Juan (2009-09-01). "Domoic acid production by Pseudo-nitzschia australis and Pseudo-nitzschia calliantha isolated from North Chile". Harmful Algae. 8 (6): 938–945. doi:10.1016/j.hal.2009.05.005. hdl:10533/197729. ISSN 1568-9883.
17. Moore, Bradley S.; Allen, Andrew E.; Hutchins, David A.; Smith, G. Jason; Oborník, Miroslav; Luhavaya, Hanna; Bielinski, Vincent A.; Bertrand, Erin M.; Miles, Zachary D. (2018-09-28). "Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom". Science. 361 (6409): 1356–1358. Bibcode:2018Sci...361.1356B. doi:10.1126/science.aau0382. ISSN 0036-8075. PMC 6276376. PMID 30262498.
18. Haschek, Wanda; Rousseaux, Colin (2013). Haschek and Rousseaux's Handbook of Toxicologic Pathology. Elsevier Science & Technology. pp. 1159–1162. ISBN 9780124157590.
19. Brunson, John K.; McKinnie, Shaun M. K.; Chekan, Jonathan R.; McCrow, John P.; Miles, Zachary D.; Bertrand, Erin M.; Bielinski, Vincent A.; Luhavaya, Hanna; Oborník, Miroslav (2018-09-28). "Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom". Science. 361 (6409): 1356–1358. Bibcode:2018Sci...361.1356B. doi:10.1126/science.aau0382. ISSN 0036-8075. PMC 6276376. PMID 30262498.
20. Bargu, Sibel; Powell, Christine L.; Wang, Zhihong; Doucette, Gregory J.; Silver, Mary W. (2008-01-01). "Note on the occurrence of Pseudo-nitzschia australis and domoic acid in squid from Monterey Bay, CA (USA)". Harmful Algae. 7 (1): 45–51. doi:10.1016/j.hal.2007.05.008. ISSN 1568-9883.
21. "Domoic Acid Poisoning". Northwest Fisheries Science Center. Retrieved June 7, 2019.
22. Pulido, Olga M. (2008). "Domoic Acid Toxicologic Pathology: A Review". Marine Drugs. 6 (2): 180–219. doi:10.3390/md20080010. ISSN 1660-3397. PMC 2525487. PMID 18728725.