In 2008, Mount Olive won the NCAA Division II Baseball National Championship. The Trojans posted a 58–6 record that year, winning the Conference Carolinas and NCAA II South Atlantic Regional titles. The Trojans defeated Ouachita Baptist (Ark.) 6–5 in the first round of the National Finals and Ashland (Ohio) 18–7 in the second round. Mount Olive defeated Central Missouri 5–3 in the semifinal round and claimed its first-ever national championship with a 6–2 victory over Ouachita Baptist in the title game. The national championship game was televised live on CBS College Sports. The National Finals took place in Sauget, Illinois.
Mount Olive teams have made 30 NCAA Division II tournament appearances.
Mount Olive teams have won a combined 39 Conference Carolinas regular season and/or tournament championships.
Mount Olive was the recipient of the 2011–12 Joby Hawn Cup, awarded to the top athletics program in Conference Carolinas. In addition to the overall award, Mount Olive also captured the Men's Sports Hawn Cup and the Women's Sports Hawn Cup.