Grillo is from Astoria, Queens.[2] She began her career as community relations specialist for the New York City School Construction Authority. Grillo later served in several senior roles in the department before being appointed as CEO and president in 2014. On July 16, 2018, she was appointed commissioner of the New York City Department of Design and Construction.[3] In December 2021, Mayor-elect Eric Adams announced that he had selected Grillo to serve as first deputy mayor. She assumed office in January 2022.[4] Grillo succeeded deputy mayor Dean Fuleihan.[2]
Adams replaced Grillo with close associate Sheena Wright, who resigned in October 2024.