The preserve protects a type of natural community known as a "shale barrens". Shale barrens are typified by thin soils on south-facing slopes, and feature hot, dry conditions. They are rare in the eastern United States; within Virginia, this type of landscape is restricted largely to the Ridge and Valley region.[3] Several rare plants are found at Johnsons Creek Natural Area Preserve, including the shale-barren rockcress (Boechera serotina).[2]
Johnsons Creek Natural Area Preserve was initially purchased by The Nature Conservancy, and was dedicated as a Natural Area Preserve in 1990.[3] The preserve is owned and maintained by the Virginia Department of Conservation and Recreation. It does not include improvements for public access, and visitors must make arrangements with a state-employed land steward prior to visiting.[2]