Guinea-Bissau competed at the 2012 Summer Olympics in London, from 27 July to 12 August 2012. This was the nation's fifth consecutive appearance at the Olympics.
Four athletes from Guinea-Bissau were selected to the team, competing only in athletics and freestyle wrestling. Half of its team had competed in Beijing, including sprinter Holder da Silva and freestyle wrestler Augusto Midana; both of them advanced past the first round of their events. Midana reprised his role to be the nation's flag bearer at the opening ceremony. Guinea-Bissau won no medals at the games.
