Bacteroides fragilis

Bacteroides fragilis
Scientific classification
Domain:	Bacteria
Phylum:	Bacteroidota
Class:	Bacteroidia
Order:	Bacteroidales
Family:	Bacteroidaceae
Genus:	Bacteroides
Species:	B. fragilis
Binomial name
Bacteroides fragilis (Veillon and Zuber 1898) Castellani and Chalmers 1919 (Approved Lists 1980)

Bacteroides fragilis is an anaerobic, Gram-negative, pleomorphic to rod-shaped bacterium. It is part of the normal microbiota of the human colon and is generally commensal,^[1][2] but can cause infection if displaced into the bloodstream or surrounding tissue following surgery, disease, or trauma.^[3]

Habitat

Bacteroides fragilis resides in the human gastrointestinal tract and is essential to healthy gastrointestinal function such as mucosal immunity and host nutrition.^[4] As a mesophile, optimal growth occurs at 37 °C and a pH around 7.^[5][4]

Morphology

Cells of B. fragilis are rod-shaped to pleomorphic with a cell size range of 0.5–1.5 × 1.0–6.0 μm.^[4]B. fragilis is a Gram-negative bacterium and does not possess flagella or cilia making it immotile. However, it does utilize peritrichous fimbriae for adhesion to other molecular structures. B. fragilis also utilizes a complex series of surface proteins, lipopolysaccharide chains, and outer membrane vesicles to help survive the volatile intestinal micro-environment.^[6]

Metabolism and mutualism in the gut microbiome

B. fragilis is an aerotolerant, anaerobic chemoorganotroph capable of fermenting a wide variety of glycans available in the human gut microenvironment including glucose, sucrose, and fructose. B. fragilis can also catabolize a variety of biopolymers, polysaccharides, and glycoproteins into smaller molecules which can then be used and further broken down by other microbes. Fatty acids produced by the fermentation of carbohydrates can serve as a source of energy for the host.^[6][4] Cytochrome bd oxidase is essential for oxygen consumption in B. fragilis and can allow other obligate anaerobes to survive in the now oxygen-reduced microenvironment.^[7][6] Animals lacking gut bacteria require 30% more caloric intake to maintain body mass.^[6]

Environment-sensing systems

The complex environmental-sensory system allows B. fragilis to survive and adapt in the ever-changing human gut microbiome. This system is composed of many components and can effectively handle a variety of threats to the bacteria.

Bacteriocins

B. fragilis intestinal isolates secrete high levels of bacteriocin proteins and are resistant to other bacteriocins secreted by other closely related isolates. This mechanism is believed to reduce the level of intra-specific competition.^[4]

Bile salt resistance

B. fragilis utilizes enzymes such as bile salt hydrolase to resist the degrading effects of bile salts. Detergent activity of bile salts can permeabilize bacterial membranes which can eventually lead to membrane collapse and/or cell damage.^[4]

Oxidative stress response

Proteins such as catalase, superoxide dismutase, and alkyl hydroperoxide reductase protect the organism from harmful oxygen radicals. This permits growth in the presence of nanomolar concentrations of O₂.^[4]

Antibiotic resistance

Member of the genus Bacteroides are characterized with having the highest numbers of antibiotic resistance mechanisms accompanied by the highest resistance rates amongst anaerobic bacteria. The high resistance to antibiotics of B.fragilis is mainly attributed to genetic plasticity.^[8] Species of the Bacteroidaceae have displayed increasing resistance to antimicrobial agents such as cefoxitin, clindamycin, metronidazole, carbapenems, and fluoroquinolones.^[6][4]

Resistance reservoirs

Bacteroides species accumulate a variety of antibiotic/antimicrobial resistance genes as they reside in the gastrointestinal tract. This allows the genetic transfer of these genes to other Bacteroides species and possibly other more virulent bacteria leading to an overall increase in multi-drug resistance. This is exacerbated by the tendency of resistance genes to be relatively stable even without the presence of the antibiotic.^[6]

Epidemiology and pathogenesis

The B. fragilis group is the most commonly isolated Bacteroidaceae in anaerobic infections, especially those that originate from the gastrointestinal microbiota. B. fragilis is the most prevalent organism in the B. fragilis group, accounting for 41% to 78% of the isolates of the group. These organisms are resistant to penicillin by virtue of production of beta-lactamase, and by other unknown factors.^[9]

This group was formerly classified as subspecies of B. fragilis (i.e. B. f. ssp. fragilis, B. f. ssp. distasonis, B. f. ssp. ovatus, B. f. ssp. thetaiotaomicron, and B. f. ssp. vulgatus). They have been reclassified into distinct species on the basis of DNA homology studies.^[10] B. fragilis (formerly known as B. f. ssp. fragilis) is often recovered from blood, pleural fluid, peritoneal fluid, wounds, and brain abscesses.^{[citation needed]}

Although the B. fragilis group is the most common species found in clinical specimens, it is the least common Bacteroides present in fecal microbiota, comprising only 0.5% of the bacteria present in stool. Their pathogenicity partly results from their ability to produce capsular polysaccharide, which is protective against phagocytosis^[6] and stimulates abscess formation.^[3]

Bacteroides fragilis is involved in 90% of anaerobic peritoneal infections.^[11] It also causes bacteremia^[12] associated with intra-abdominal infections, peritonitis and abscesses following rupture of viscus, and subcutaneous abscesses or burns near the anus.^[13] Though it is gram negative, it has an altered LPS and does not cause endotoxic shock. Untreated B. fragilis infections have a 60% mortality rate.^[6]

Anti-inflammatory effects

B. fragilis polysaccharide A (PSA) has been shown to protect animals from experimental diseases like colitis, asthma, or pulmonary inflammation.^[14] B. fragilis mutants lacking surface polysaccharides cannot easily colonize the intestine.^[8] PSA colonization of B. fragilis in the gut mucosa induces regulatory T cells and suppresses pro-inflammatory T helper 17 cells.^[14]

See also

• List of oncogenic bacteria
• Infectious causes of cancer
• Pathogenic bacteria

References

1. Kuwahara T, Yamashita A, Hirakawa H, Nakayama H, Toh H, Okada N, et al. (October 2004). "Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation". Proceedings of the National Academy of Sciences of the United States of America. 101 (41): 14919–14924. Bibcode:2004PNAS..10114919K. doi:10.1073/pnas.0404172101. PMC 522005. PMID 15466707.
2. "Bacteroides fragilis". Johns Hopkins ABX Guide.
3. Levinson W (2010). Review of Medical Microbiology and Immunology (11th ed.).
4. Wexler HM (2014). "The Genus Bacteroides". In Rosenberg E, DeLong EF, Lory S, Stackebrandt E (eds.). The Prokaryotes: Other Major Lineages of Bacteria and The Archaea (Fourth ed.). Heidelberg: Springer. pp. 459–484. doi:10.1007/978-3-642-38954-2_129. ISBN 978-3-642-38954-2.
5. "BacMap". bacmap.wishartlab.com. Retrieved 2021-11-13.
6. Wexler HM (October 2007). "Bacteroides: the good, the bad, and the nitty-gritty". Clinical Microbiology Reviews. 20 (4): 593–621. doi:10.1128/CMR.00008-07. PMC 2176045. PMID 17934076.
7. Baughn AD, Malamy MH (January 2004). "The strict anaerobe Bacteroides fragilis grows in and benefits from nanomolar concentrations of oxygen". Nature. 427 (6973): 441–444. Bibcode:2004Natur.427..441B. doi:10.1038/nature02285. PMID 14749831. S2CID 4420207.
8. Sun F, Zhang Q, Chen W (2019). "A potential species of next-generation probiotics? The dark and light sides of Bacteroides fragilis in health". Food Research International. 126: 108590. doi:10.1016/j.foodres.2019.108590. PMID 31732047. S2CID 201203450.
9. Snydman DR, Jacobus NV, McDermott LA, Golan Y, Hecht DW, Goldstein EJ, et al. (January 2010). "Lessons learned from the anaerobe survey: historical perspective and review of the most recent data (2005-2007)". Clinical Infectious Diseases. 50 (Suppl 1): S26 – S33. doi:10.1086/647940. PMID 20067390.
10. Baron EJ, Allen SD (June 1993). "Should clinical laboratories adopt new taxonomic changes? If so, when?". Clinical Infectious Diseases. 16 (Suppl 4): S449 – S450. doi:10.1093/clinids/16.Supplement_4.S449. PMID 8324167.
11. Bacteroides infections at eMedicine
12. Brook I (June 2010). "The role of anaerobic bacteria in bacteremia". Anaerobe. 16 (3): 183–189. doi:10.1016/j.anaerobe.2009.12.001. PMID 20025984.
13. Brook I (October 2008). "Microbiology and management of abdominal infections". Digestive Diseases and Sciences. 53 (10): 2585–2591. doi:10.1007/s10620-007-0194-6. PMID 18288616. S2CID 35945399.
14. Erturk-Hasdemir D, Ochoa-Repáraz J, Kasper DL, Kasper LH (2021). "Exploring the Gut-Brain Axis for the Control of CNS Inflammatory Demyelination: Immunomodulation by Bacteroides fragilis' Polysaccharide A". Frontiers in Immunology. 12: 662807. doi:10.3389/fimmu.2021.662807. PMC 8131524. PMID 34025663.

External links

• Bacteroides references in Baron's Medical Microbiology (online at the NCBI bookshelf).
• Type strain of Bacteroides fragilis at BacDive - the Bacterial Diversity Metadatabase