The competition continued to be played in two stages, but the number of teams being reduced from 20 to 16.[4] In the first stage, the 16 clubs will be divided in two series (North and South) of 8 teams, according to geographic criteria. In each series, teams play against each other once in a single round-robin system.
In the second stage, the four best-placed teams of each of the Series advanced to the championship group and the remaining teams to the relegation series. On the championship group, all eight teams play against each other in a home-and-away double round-robin system, to decide the champions. On the relegation series (North and South) the competition work in the same way, with the first and second placed teams being granted a place in 2022–23 Campeonato Nacional de Futebol Feminino. The eighth-placed team (last) was relegated while the teams place from third to seventh will play the relegation play-offs along with teams from Campeonato Nacional II Divisão placed second to fourth. Eight teams participate in the play-off, with the winner of each round advancing to the next one. Only the winner guarantees a place in 2022–23 Campeonato Nacional de Futebol Feminino.