The 2016 Regional League Division 2 (also known as the AIS League Division 2 for sponsorship reasons) was the 11th season of the Regional League Division 2, it had redirected from the division 2, since its establishment in 2006. The 94 clubs will be divided into 8 groups (regions).
Locations of the 2016 Regional League Division 2 All Teams 2016 Regional League Division 2 Bangkok & field Region 2016 Regional League Division 2 Bangkok & Eastern Region 2016 Regional League Division 2 Central Region 2016 Regional League Division 2 Eastern Region 2016 Regional League Division 2 Western Region 2016 Regional League Division 2 Northern Region 2016 Regional League Division 2 North Eastern Region 2016 Regional League Division 2 Southern Region
Following the death of King Bhumibol Adulyadej, the Football Association of Thailand cancelled the remaining league season on 14 October 2016. Last 4 teams of the 2016 Regional League Division 2 Champions League round had drawn to ranked for promoted to the 2017 Thai League Division 1. They had ranked on October 20, 2016.