The 1965 Campeonato Ecuatoriano de Fútbol (English: Ecuadorian Football Championship) was the 7th national championship for football teams in Ecuador. Emelec won their third national title. They qualified to the 1966 Copa Libertadores along with 9 de Octubre.
Qualified teams
The number of teams remained the same at eight. The qualified teams included the top-four finishers from the Campeonato Interandino and the Campeonato de Guayaquil. Universidad Católica made their first appearance in the tournament.
Source: [1] Legend: Blue = home team win; Yellow = draw; Red = away team win. Notes:
^Teams from the same association did not play each other.
^ abcdAucas lost the matches by fielding an ineligible player.
Runner-up playoff
Since 9 de Octubre, Barcelona, and Patria finished the tournament equal on points, a playoff was needed to determine who finished as the runner-up and qualified to the 1966 Copa Libertadores. Patria declined to participate. After tying at a goal apiece, 9 de Octubre was declared the runner-up since they had a better goal average throughout the season (1.37 v. 1.36).